Recombinant Human CD59 protein (ab134887)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: = 5.000 Eu/µg
- Tags: His tag N-Terminus, T7 tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human CD59 protein
CD59 タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
ab134887 was refolded using temperature shift inclusion body refolding technology and chromatographically purified. -
エンドトキシン・レベル
= 5.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MASMTGGQQMGRGHHHHHHGNLYFQGGEFALVQCYNCPNPTADCKTAVNC SSDFDACLITKAGLQVYNKCWKFEHCNFNDVTTRLRENELTYYCCKKDLC NFNEQLEN -
予測される分子量
12 kDa including tags -
領域
26 to 102 -
タグ
His tag N-Terminus , T7 tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab134887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Proprietary formulation of NaCl, KCl, EDTA, Arginine, DTT and Glycerol.
関連情報
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別名
- 16.3A5
- 1F5
- 1F5 antigen
see all -
機能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
関連疾患
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
配列類似性
Contains 1 UPAR/Ly6 domain. -
翻訳後修飾
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
細胞内局在
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
画像
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SDS PAGE analysis of ab134887 (lanes 4-6), using BSA as a reference protein (lanes 1-3).
Lane 1: 10µg BSA
Lane 2: 5µg BSA
Lane 3: 1µg BSA
Lane 4: 10µl ab134887
Lane 5: 5µl ab134887
Lane 6: 1µl ab134887
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SDS PAGE analysis of ab134887 (lanes 4-5), using BSA as a reference protein (lanes 1-3). 4-20% gradient gel was used.
Lane 1: 10µl BSA
Lane 2: 5µl BSA
Lane 3: 1µl BSA
Lane 4: 20µl ab134887 (Stored at -20°C for 180 days)
Lane 5: 20µl ab134887 (Stored at -20°C, then shift to 4°C for 30 days)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab134887 は論文での使用が確認できていません。