Recombinant human CD47 protein (Active) (ab271439)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human CD47 protein (Active)
CD47 タンパク質・ペプチド 製品一覧 -
生理活性
Assay Description: Coated wells in assay plate with either PBS (No Coat) or 250 ng/well of CD47-His in 50 μl PBS (overnight at 4 deg). Mixed various concentrations of SIRPα -biotin (0-3000nM) in assay buffer and added to assay plate wells. Incubated for 2h at RT. Detected binding with Strep-HRP generated luminescence. Assay Buffer is PBS + 0.1% BSA.
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精製度
>= 90 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS P -
予測される分子量
15 kDa including tags -
分子量情報
This protein runs at a higher molecular weight SDS-PAGE due to glycosylation. -
領域
19 to 139 -
タグ
His tag C-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab271439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Antigen identified by monoclonal antibody 1D8
- Antigenic surface determinant protein OA3
- CD 47
see all -
機能
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. -
組織特異性
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant human CD47 protein (Active) (ab271439)
SDS-PAGE analysis of 2 µg ab271439.
This protein runs at a higher molecular weight due to glycosylation.
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Functional Studies - Recombinant human CD47 protein (Active) (ab271439)CD47:SIRPα binding activity of ab271439.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (0)
ab271439 は論文での使用が確認できていません。
