Recombinant human CD47 protein (Active) (ab220594)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human CD47 protein (Active)
CD47 タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 2-13 ng/ml.
Immobilized Anti-CD47 MAb, Human IgG4 at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 0.05-2.0 ng/ml.
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTF DGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTE LTREGETIIELKYRVVSWFSP -
予測される分子量
17 kDa including tags -
領域
19 to 139 -
タグ
His tag C-Terminus , Avi tag C-Terminus -
配列の追加情報
NP_942088.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab220594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
After reconstitution this product is stable for 3 months at -80°C under sterile conditions.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 10% Trehalose, 0.08% Bicine
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
関連情報
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別名
- Antigen identified by monoclonal antibody 1D8
- Antigenic surface determinant protein OA3
- CD 47
see all -
機能
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. -
組織特異性
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
画像
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Immobilized Human SIRP alpha, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 2-13 ng/ml.
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SDS-PAGE analysis of reduced ab220594 stained overnight with Coomassie Blue.
The protein migrates as 33-44 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
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Immobilized Anti-CD47 MAb, Human IgG4 at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 0.05-2.0 ng/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab220594 は論文での使用が確認できていません。