Recombinant Human CD4 protein (His tag) (ab270593)

Submit a review Submit a question
SDS-PAGE - Recombinant Human CD4 protein (His tag) (ab270593)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 80% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human CD4 protein (His tag)
      CD4 タンパク質・ペプチド 製品一覧

    • 精製度

      > 80 % SDS-PAGE.

    • エンドトキシン・レベル

      < 1.000 Eu/µg

    • 発現系

      Escherichia coli

    • アクセッション番号

      P01730

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSK LNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGL TANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLE LQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPL AFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMG KKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKN LTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDS GQVLLESNIKVLPTW

      • 予測される分子量

        51 kDa including tags

      • 実際の分子量

        44 kDa including tags

      • 領域

        26 to 390

      • タグ

        His tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab270593 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

    • 製品の状態

      Lyophilized
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.01% Sarkosyl, 5% Trehalose

    • 再構成
      Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

    関連情報

    • 別名

      • CD 4
      • CD4
      • CD4 (L3T4)
      • CD4 antigen
      • CD4 antigen (p55)
      • CD4 molecule
      • CD4 receptor
      • CD4+ Lymphocyte deficiency, included
      • CD4_HUMAN
      • CD4mut
      • L3T4
      • Leu3
      • Ly-4
      • Lymphocyte antigen CD4
      • MGC165891
      • OTTHUMP00000238897
      • p55
      • T cell antigen T4
      • T cell antigen T4/LEU3
      • T cell differentiation antigen L3T4
      • T cell OKT4 deficiency, included
      • T cell surface antigen T4/Leu 3
      • T cell surface antigen T4/Leu3
      • T cell surface glycoprotein CD4
      • T-cell surface antigen T4/Leu-3
      • T-cell surface glycoprotein CD4
      • W3/25
      • W3/25 antigen
      see all

    • 機能

      Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

    • 配列類似性

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • 翻訳後修飾

      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

    • 細胞内局在

      Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human CD4 protein (His tag) (ab270593)
      SDS-PAGE - Recombinant Human CD4 protein (His tag) (ab270593)

      Recombinant human CD4 protein fragment (ab270593) resolved on a SDS-PAGE gel under reducing conditions.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270593 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270593 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab270593.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com