Bioactive grade

Recombinant human CD4 protein (Active) (ab167756)

製品の詳細

  • 製品名

    Recombinant human CD4 protein (Active)
    CD4 タンパク質・ペプチド 製品一覧
  • 生理活性

    Measured by its ability to bind with HIV-1 gp120 in a functional ELISA.

  • 精製度

    > 95 % SDS-PAGE.

  • エンドトキシン・レベル

    < 1.000 Eu/µg
  • 発現系

    HEK 293 cells
  • アクセッション番号

  • タンパク質長

    Protein fragment
  • Animal free

    No
  • 由来

    Recombinant
    • 生物種

      Human
    • 配列

      KKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTKGPSK LNDRADSRRS LWDQGNFPLI IKNLKIEDSD TYICEVEDQK EEVQLLVFGL TANSDTHLLQ GQSLTLTLES PPGSSPSVQC RSPRGKNIQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ KASSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWITFD LKNKEVSVKR VTQDPKLQMG KKLPLHLTLP QALPQYAGSG NLTLALEAKT GKLHQEVNLV VMRATQLQKN LTCEVWGPTS PKLMLSLKLE NKEAKVSKRE KAVWVLNPEA GMWQCLLSDS GQVLLESNIK VLPTWSTPVQ P
    • 予測される分子量

      68 kDa including tags
    • 領域

      26 to 396
    • タグ

      Fc tag C-Terminus
    • 配列の追加情報

      Recombinant Human CD4 Protein fused with Fc fragment of human IgG1 at the C-terminus. (AAH25782)

特性

Our Abpromise guarantee covers the use of ab167756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    ELISA

  • 製品の状態

    Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.5
    Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris

    Lyophilized from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    It is recommended to reconstitute the lyophilized protein in sterile deionized water. Solubilise for 30 to 60 minutes at room temperature with occasional gentle mixing.

関連情報

  • 別名

    • CD 4
    • CD4
    • CD4 (L3T4)
    • CD4 antigen
    • CD4 antigen (p55)
    • CD4 molecule
    • CD4 receptor
    • CD4+ Lymphocyte deficiency, included
    • CD4_HUMAN
    • CD4mut
    • L3T4
    • Leu3
    • Ly-4
    • Lymphocyte antigen CD4
    • MGC165891
    • OTTHUMP00000238897
    • p55
    • T cell antigen T4
    • T cell antigen T4/LEU3
    • T cell differentiation antigen L3T4
    • T cell OKT4 deficiency, included
    • T cell surface antigen T4/Leu 3
    • T cell surface antigen T4/Leu3
    • T cell surface glycoprotein CD4
    • T-cell surface antigen T4/Leu-3
    • T-cell surface glycoprotein CD4
    • W3/25
    • W3/25 antigen
    see all
  • 機能

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 配列類似性

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 細胞内局在

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt

画像

  • Reduced ab167756 on SDS-PAGE, stained overnight with Coomassie Blue.

    Purity of protein >95%.

    The protein migrates as 76 kDa under reducing condition due to glycosylation.

参考文献

ab167756 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab167756.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録