Recombinant human CD38 protein (Active) (APC) (ab269982)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human CD38 protein (Active) (APC)
CD38 タンパク質・ペプチド 製品一覧 -
生理活性
Assay conditions: Add CD38 enzyme to 50 μl reaction mix containing NGD+ (Nicotinamide Guanine Dinucleotide). Incubate 4 min at room temperature. Read fluorescence (λ exc=300 nm, λ em=410 nm).
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精製度
> 90 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGA FISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDM FTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTV SRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWV IHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPE DSSCTSEI -
予測される分子量
31 kDa including tags -
領域
43 to 300 -
タグ
His tag C-Terminus -
配列の追加情報
Labeled with APC. NM_001775
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標識
APC. Ex: 645nm, Em: 660nm
関連製品
特性
Our Abpromise guarantee covers the use of ab269982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Acute lymphoblastic leukemia cells antigen CD38
- ADP ribosyl cyclase
- ADP ribosyl cyclase 1
see all -
機能
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
組織特異性
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
配列類似性
Belongs to the ADP-ribosyl cyclase family. -
発生段階
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
細胞内局在
Membrane. - Information by UniProt
画像
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Functional Studies - (ab269982)
Functional analysis of ab269982.
Add CD38 enzyme to 50 μl reaction mix containing NGD+ (Nicotinamide Guanine Dinucleotide). Incubate 4 min at room temperature. Read fluorescence (λ exc=300 nm, λ em=410 nm).
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SDS-PAGE - (ab269982)SDS-PAGE analysis of ab269982 ona 4-20% gel with Coomassie staining.
Lane 1: ab269982 (8 μg).
Lane 2: Unlabeled CD38 (4 μg).
Lane 3: APC (4 μg).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab269982 は論文での使用が確認できていません。
