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Recombinant human CCL14 protein (ab9846)

Submit a review Q&A (2)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 99% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

製品の詳細

  • 製品名

    Recombinant human CCL14 protein
    CCL14 タンパク質・ペプチド 製品一覧

  • 精製度

    > 99 % SDS-PAGE.
    Sterile filtered Greater than 99% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).

  • 発現系

    Escherichia coli

  • アクセッション番号

    Q16627

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN

    • 予測される分子量

      59 kDa

特性

Our Abpromise guarantee covers the use of ab9846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Lyophilized

  • 備考

    The biological activity of this product is determined by its ability to chemoattract human monocytes using a concentration range of 5.0-20.0 ng/ml.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    For lot specific reconstitution information please contact our Scientific Support Team.

関連情報

  • 別名

    • CC-1
    • CC-3
    • CCL14
    • CCL14_HUMAN
    • chemokine (C-C motif) ligand 14
    • Chemokine CC-1/CC-3
    • chemokine CC1
    • chemokine CC3
    • chemokine HCC1
    • chemokine HCC3
    • CKb1
    • HCC 1
    • HCC 3
    • HCC-1(1-74)
    • HCC-1(9-74)
    • HCC-1/HCC-3
    • HCC-3
    • HCC1
    • HEMOFILTRATE CC CHEMOKINE 1
    • MCIF
    • NCC-2
    • NCC2
    • new CC chemokine 2
    • SCYA14
    • SCYL2
    • small inducible cytokine subfamily A (Cys-Cys), member 14
    • Small-inducible cytokine A14
    • SY14
    see all

  • 機能

    Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.

  • 組織特異性

    Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.

  • 配列類似性

    Belongs to the intercrine beta (chemokine CC) family.

  • 翻訳後修飾

    The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.
    HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids.

  • 細胞内局在

    Secreted.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab9846 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9846 は論文での使用が確認できていません。

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

I was planning to buy the HCC1 recombinant protein from you. Before that I want to clarify one thing. On HCC1 protein web page on abcam's site there must be some kind of mistake. You write that HCC1 (CAPER) protein is 72AA long while it's 530 or 373AA depending on the izoform and you give the AA sequence for chemokine CC-1 which is completely different protein. Could you please confirm which of these two proteins are you selling under the product number ab9846.

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Answer

Thank you for your enquiry. Yes, you are correct this recombinant protein has been incorrectly listed in the relevance section, the swissprot links and the research area on the datasheet of ab9846. Please accept my apolagies. The recombinant protein corresponds to chemokine CC-1 with the synonyms Hemofiltrate CC Chemokine-1, CCL14, HCC-1/HCC-3 and NCC-2. As far as I understand this is a significantly different protein to the HCC1 that you are interested in. Therefore I can tell you that it does not relate to an RNA binding and possible splicing factor target but a protein that chemoattracts blood monocytes. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

I have a problem with detecting your HCC-1 recombinant protein (Cat# ab9846) with your polyclonal Anti-HCC-1 antibody (Cat#ab9845) by western blotting. Could you help me with my problem? Maybe you could forward this mail to somebody who could help me. My protocol was as follows: I have separated 10 ng HCC-1/lane on a SDS-PAGE. Semi-dry blotting to a Hybond-P PVDF membrane, blocked with non-fat milk, primary Ab: 1 ug/ml anti-HCC1 (Cat#ab9845), Secondary Ab: my normal secondary Ab against rabbit, and detected with Supersignal West Dura substare from Pierce. However, I still do not get any band????? Hope you can help me,

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Answer

I have checked the datasheet for ab9845 -- Rabbit polyclonal to human HCC 1 and note the antibody can be used at a concentration of 0.1 - 0.2 µg/ml and that the detection limit for hHCC-1 is 1.5 - 3.0 ng/lane. You mention that you have used the antibody at a concentration of 1µg/ml (1:500 dilution) using 10 ng of protein. Strictly speaking, you should be able to detect a a band. My advice would be to run another blot loading more protein on your gel - I'd recommend that you try loading 25ng and 50ng of the recombinant protein and if you still can't detect a band that please get back to us. You could also try using the antibody at a higher concentration but 1:500 should be fine.

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