Recombinant human Caspase-9 protein (Tagged) (ab198061)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 56% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human Caspase-9 protein (Tagged)
Caspase-9 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 0.11 pmol/min/μg.
Assay conditions: 50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 10 μM Caspase 9 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em505 nm.
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精製度
>= 56 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MDEADRRLLRRCRLRLVEELQVDQLWDALLSRELFRPHMIEDIQRAGSGS RRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASFLRTNRQAAKLSK PTLENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESLRGNAD LAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFM VEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAV YGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVAS TSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPG FVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQM PGCFNFLRKKLFFKTSHHHHHH -
領域
1 to 416 -
タグ
His tag C-Terminus -
配列の追加情報
Procaspase- 9 is autocleaved between the prodomain and large protease subunit (p35). The cleavage product, contains both the large (p35) and small (p10 [His-tag]) protease subunits. MWt: 35 & 10 kDa
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab198061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.54% Imidazole
Constituents: 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine), 0.02% Potassium chloride, 0.64% Sodium chloride, 0.61% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- APAF-3
- APAF3
- Apoptosis related cysteine peptidase
see all -
機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
組織特異性
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
配列類似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
発生段階
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
翻訳後修飾
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
画像
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Activity assay using ab198122.
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4-20% SDS-PAGE analysis of 2.5 µg ab198061 with Coomassie staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab198061 は 2 報の論文で使用されています。
- He S et al. Dapagliflozin Protects Methamphetamine-Induced Cardiomyopathy by Alleviating Mitochondrial Damage and Reducing Cardiac Function Decline in a Mouse Model. Front Pharmacol 13:925276 (2022). PubMed: 35873593
- Xu WF et al. Gasdermin E-derived caspase-3 inhibitors effectively protect mice from acute hepatic failure. Acta Pharmacol Sin N/A:N/A (2020). PubMed: 32457417