Recombinant human Caspase-6/CASP-6 protein (Active) (ab198067)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Caspase-6/CASP-6 protein (Active)
Caspase-6/CASP-6 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 1720 pmol/min/μg.
Assay conditions: 50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 20 μM Caspase 6 / CASP-6 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em505 nm. -
精製度
> 90 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIF NHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKI HEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHS LVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTL PAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLT LVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLH FFPKSNHHHHH H -
領域
1 to 293 -
タグ
His tag C-Terminus -
配列の追加情報
Pro-caspase-6 is activated by autocleavage to yield 20 kDa and 18 kDa proteins (large subunit) and an 11 kDa small subunit (His-tag) that dimerize to form the active enzyme.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab198067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
Previously labelled as Caspase-6.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 2.18% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.01% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Apoptotic protease Mch-2
- Apoptotic protease MCH2
- CASP-6
see all -
機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. -
細胞内局在
Cytoplasm. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab198067 は論文での使用が確認できていません。