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Recombinant human Casein Kinase 1 gamma 2 protein (ab104025)

Submit a review Q&A (1)
Functional Studies - Recombinant human Casein Kinase 1 gamma 2 protein (ab104025)
  • SDS-PAGE - Recombinant human Casein Kinase 1 gamma 2 protein (ab104025)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 80% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, WB

製品の詳細

  • 製品名

    Recombinant human Casein Kinase 1 gamma 2 protein
    Casein Kinase 1 gamma 2 タンパク質・ペプチド 製品一覧

  • 生理活性

    The Specific activity of ab104025 was determined to be 30 nmol/min/mg.

  • 精製度

    > 80 % SDS-PAGE.
    Purity was determined to be >85% by densitometry. Affinity purified.

  • 発現系

    Baculovirus infected Sf9 cells

  • アクセッション番号

    P78368

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 予測される分子量

      73 kDa including tags

    • 領域

      1 to 415

特性

Our Abpromise guarantee covers the use of ab104025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    Western blot

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名

    • Casein kinase 1, gamma 2
    • Casein kinase I isoform gamma 2
    • Casein kinase I isoform gamma-2
    • CK1g2
    • CKI gamma 2
    • CKI-gamma 2
    • CSNK1G 2
    • Csnk1g2
    • KC1G2_HUMAN
    see all

  • 機能

    Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.

  • 組織特異性

    Testis.

  • 配列類似性

    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Autophosphorylated.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

画像

  • Functional Studies - Recombinant human Casein Kinase 1 gamma 2 protein (ab104025)
    Functional Studies - Recombinant human Casein Kinase 1 gamma 2 protein (ab104025)
    The Specific activity of ab104025 was determined to be 30 nmol/min/mg.
  • SDS-PAGE - Recombinant human Casein Kinase 1 gamma 2 protein (ab104025)
    SDS-PAGE - Recombinant human Casein Kinase 1 gamma 2 protein (ab104025)
    SDS-PAGE showing ab104025 at approximately 73kDa.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab104025 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab104025 は論文での使用が確認できていません。

レビューと Q&A

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Question

Hi I am interesting in purchasing your casein kinase 1 gamma 2 protein. I will be doing an assay with casein kinase 1 gamma 2 as my substrate for my protein kinase, and I need a relatively high concentration of casein kinase 1 gamma 2 protein, roughly around 0.4 mg/ml - 1.0 mg/ml. Do you have anything in that concentration range which I can purhcase? In addition, I would like to get the protein sequence of the casein kinase 1 gamma 2 that you are selling as verification. Thank you for your help.  

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Answer

Thank you for your inquiry. The only Casein Kinase 1 gamma 2 protein that we carry in the catalogue is ab104025, the protein you found. The concentration of this protein is 0.1 mg/ml. If you need to make a bulk order purchase (of 5 or more units), you can email my colleague Andrea at sales@abcam.com. The protein sequence is full-length recombinant Casein Kinase 1 gamma 2 expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM 001319. MDFDKKGGKGETEEGRRMSKAGGGRSSHGIRSSGTSSGVLMVGPNFRVGKKIGCGNFGEL RLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLSATEGVPQVYYFGPCGKYNAMVLE LLGPSLEDLFDLCDRTFTLKTVLMIAIQLITRMEYVHTKSLIYRDVKPENFLVGRPGTKR QHAIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGH MFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRLDFF EKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPSQPQLRDKTQPHSK NQALNSTNGELNADDPTAGHSNAPITAPAEVEVADETKCCCFFKRRKRKSLQRHK I hope this information helps. Please contact us with any other questions.

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