Recombinant Human beta I Tubulin protein (ab164310)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: ELISA, WB
製品の詳細
-
製品名
Recombinant Human beta I Tubulin protein -
発現系
Wheat germ -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
MREIVHIQIGQCGNQIGAKFWEMIGEEHGIDLAGSDRGASALQLERISVY YNEAYGRKYVPRAVLVDLEPGTMDSIRSSKLGALFQPDSFVHGNSGAGNN WAKGHYTEGAELIENVLEVVRHESESCDCLQGFQIVHSLGGGTGSGMGTL LMNKIREEYPDRIMNSFSVMPSPKVSDTVVEPYNAVLSIHQLIENADACF CIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGITTSLRFPGQLNADL RKLAVNMVPFPRLHFFMPGFAPLTAQGSQQYRALSVAELTQQMFDARNTM AACDLRRGRYLTVACIFRGKMSTKEVDQQLLSVQTRNSSCFVEWIPNNVK VAVCDIPPRGLSMAATFIGNNTAIQEIFNRVSEHFSAMFKRKAFVHWYTS EGMDINEFGEAENNIHDLVSEYQQFQDAKAVLEEDEEVTEEAEMEPEDKG H -
領域
1 to 451 -
タグ
GST tag N-Terminus
-
特性
Our Abpromise guarantee covers the use of ab164310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
ELISA
Western blot
-
製品の状態
Liquid -
備考
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
関連情報
-
別名
- 2810484G07Rik
- beta 1 tubulin
- Beta tubulin 1, class VI
see all -
機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
関連疾患
Defects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab164310 は 1 報の論文で使用されています。
- Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446