Recombinant human BCMA protein (Active) (ab269983)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 82% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human BCMA protein (Active)
BCMA タンパク質・ペプチド 製品一覧 -
生理活性
Assay performed using a BCMA:APRIL[B] assay kit. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein (BCMA) was added overnight at 4 deg 100 ng/well. Various amounts of biotinylated ligand (APRIL[B]) (0-20 ng) added for 2h at RT. Strep-HRP added for 1h at RT
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精製度
> 82 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
34 kDa including tags -
領域
1 to 54 -
タグ
Avi tag C-Terminus , Fc tag C-Terminus -
配列の追加情報
C-terminal Avi tag fused to human IgG1 Fc.. NM_001192
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特性
Our Abpromise guarantee covers the use of ab269983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- B cell maturation antigen
- B cell maturation factor
- B cell maturation protein
see all -
機能
Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. -
組織特異性
Expressed in mature B-cells, but not in T-cells or monocytes. -
関連疾患
Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2. -
配列類似性
Contains 1 TNFR-Cys repeat. -
細胞内局在
Cell membrane. Endomembrane system. Perinuclear Golgi-like structures. - Information by UniProt
画像
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Functional analysis of ab269983.
Assay performed using a BCMA:APRIL[B] assay kit. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein (BCMA) was added overnight at 4 deg 100 ng/well. Various amounts of biotinylated ligand (APRIL[B]) (0-20 ng) added for 2h at RT. Strep-HRP added for 1h at RT.
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SDS-PAGE analysis of ab269983 (3 μg) on a 4-20% gel with Coomassies staining.
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Aggregation analysis of ab269983.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab269983 は論文での使用が確認できていません。