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Recombinant human BCA1 protein (ab9976)

Submit a review Q&A (1)
Western blot - Recombinant human BCA1 protein (ab9976)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 98% SDS-PAGE
    • Active: Yes
    • Suitable for: WB, Functional Studies, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant human BCA1 protein
      BCA1 タンパク質・ペプチド 製品一覧

    • 精製度

      > 98 % SDS-PAGE.
      Sterile filtered Greater than 98% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).

    • 発現系

      Escherichia coli

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 予測される分子量

        13 kDa

    特性

    Our Abpromise guarantee covers the use of ab9976 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      Functional Studies

      SDS-PAGE

    • 製品の状態

      Lyophilized

    • 備考

      The biological activity of of hBCA-1 was determined by a chemotaxis assay using human B cells. Significant chemotaxis was achieved at a concentration range of 1.0-10.0 ng/ml.
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • 再構成
      For lot specific reconstitution information please contact our Scientific Support Team.

    関連情報

    • 別名

      • ANGIE
      • ANGIE2
      • B cell attracting chemokine 1
      • B cell-attracting chemokine 1
      • B lymphocyte chemoattractant
      • B-cell chemoattractant
      • B-cell-attracting chemokine 1
      • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
      • BCA-1
      • BLC
      • BLR1L
      • C-X-C motif chemokine 13
      • Chemokine (C-X-C motif) ligand 13
      • Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
      • Chemokine, CXC motif, ligand 13
      • CXC chemokine BLC
      • CXCL13
      • CXL13_HUMAN
      • SCYB13
      • Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
      • Small inducible cytokine B13
      • Small inducible cytokine subfamily B, member 13
      • Small-inducible cytokine B13
      see all

    • 機能

      Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

    • 組織特異性

      Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.

    • 配列類似性

      Belongs to the intercrine alpha (chemokine CxC) family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    画像

    • Western blot - Recombinant human BCA1 protein (ab9976)
      Western blot - Recombinant human BCA1 protein (ab9976)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab9976 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9976 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Dr Hugh Spotswood, Technical Support: Welcome to Abcam's live support service. How may I help? Visitor: Hi Visitor: I am interested in your CXCL13 Dr Hugh Spotswood, Technical Support: Great, did you have a particular catalogue product in mind? Visitor: Do you make it (human CXCL13) yourselves or do you purchase it? Visitor: ab9976 Dr Hugh Spotswood, Technical Support: Let me have a look, please give me a minute. Dr Hugh Spotswood, Technical Support: ab9976 is BCA-1 (human) recombinant protein. Is this what you are interested in? Visitor: yes. you list it as recombinant from E. coli. DO you make it yourselves? Dr Hugh Spotswood, Technical Support: We source this product. Dr Hugh Spotswood, Technical Support: What would you like to know about it? Visitor: OK, Do you do the characterization or does the source?> Dr Hugh Spotswood, Technical Support: The characterisation is performed by our source. What information would you like to know with regards its characterisation? Visitor: DO they provide Mass-spec data on the purified product? Dr Hugh Spotswood, Technical Support: I do not know, but I can certainly enquire on your behalf and get back to you by e-mail. Visitor: Thanks. Another question. Do you also source the BCA1 antibody (ab9975) from the same source? Dr Hugh Spotswood, Technical Support: Yes, do you have a question in relation to this antibody Visitor: Nothing now. I would appreciate any information you can find on the BCA-1 you source. We have been comparing from souces and find some differences. Dr Hugh Spotswood, Technical Support: Please can you tell me whether you are referring to the BCA-1 protein or the antibody? Visitor: BCA-1 protein (ab9976) Dr Hugh Spotswood, Technical Support: Yes, certainly. I will log your enquiry and get back to you by e-mail if this is Ok with you. Requested details of ab9976 - Is it characterised by mass spec?

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    Answer

    Thank you for your enquiry. I have been in touch with the source of this antibody and they have told me that every LOT of BCA1 protein (ab9976) is verified/characterized using mass spec. Should you require specific data for a particular LOT number then this is definitely something that I can arrange. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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