Recombinant Human APAF1 protein (Tagged) (ab198064)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human APAF1 protein (Tagged) -
精製度
> 90 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MHHHHHHDYKDDDDKDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLT ISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAAL LHDGIPVVSSSSVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEP GWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLM KLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWD SWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSL FVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQN KQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPT KVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTE KNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMH KELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEF LSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEW INKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETG EKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDE HSETQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSV NHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQED MEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYC DFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSS FLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRL QLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIF QSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQET VKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHD ATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGD DNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAG GYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQT LE -
予測される分子量
143 kDa including tags -
領域
2 to 1237 -
タグ
His-DDDDK tag N-Terminus -
配列の追加情報
This product is for the mature full length Isoform 2 protein from aa 2 to 1237. The initial Met is not included. GenBank Accession No. NM_013229.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab198064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
80 µg/ml DDDDK peptide
関連情報
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別名
- APAF 1
- Apaf-1
- APAF_HUMAN
see all -
機能
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
組織特異性
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
配列類似性
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
ドメイン
The CARD domain mediates interaction with APIP. -
細胞内局在
Cytoplasm. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab198064 は 1 報の論文で使用されています。
- Wang Q et al. APAF1-Binding Long Noncoding RNA Promotes Tumor Growth and Multidrug Resistance in Gastric Cancer by Blocking Apoptosome Assembly. Adv Sci (Weinh) 9:e2201889 (2022). PubMed: 35975461