Recombinant human Amphiregulin protein (ab104355)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
製品の詳細
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製品名
Recombinant human Amphiregulin protein
Amphiregulin タンパク質・ペプチド 製品一覧 -
生理活性
Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml. -
精製度
> 95 % SDS-PAGE.
Purity is > 95% by SDS-PAGE gel and HPLC analyses. Sterile filtered through a 0.2 micron filter. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEF QNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSK -
予測される分子量
11 kDa -
領域
101 to 198
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特性
Our Abpromise guarantee covers the use of ab104355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
Western blot
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製品の状態
Lyophilized -
備考
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituent: 0.082% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
関連情報
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別名
- A REG
- Amphiregulin
- Amphiregulin B
see all -
機能
Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. -
配列類似性
Belongs to the amphiregulin family.
Contains 1 EGF-like domain. -
細胞内局在
Membrane. - Information by UniProt
画像
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Western blot - Recombinant human Amphiregulin protein (ab104355)Anti-Amphiregulin antibody [MM0089-8K16] (ab89119) at 1/500 dilution +
Recombinant human Amphiregulin protein (ab104355) at 0.1 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 8 minutesab89119 recognizes the recombinant full length soluble Human Amphiregulin protein (ab104355) which has an expected molecular weight of ~12 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab104355 は 1 報の論文で使用されています。
- Hobor S et al. TGFa and amphiregulin paracrine network promotes resistance to EGFR blockade in colorectal cancer cells. Clin Cancer Res 20:6429-38 (2014). PubMed: 24916700