Recombinant human Activin Receptor Type IIA/ACVR2A protein (Active) (Biotin) (ab271359)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: = 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, Avi tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Activin Receptor Type IIA/ACVR2A protein (Active) (Biotin)
Activin Receptor Type IIA/ACVR2A タンパク質・ペプチド 製品一覧 -
生理活性
Kinase assay using casein as substrate.
Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 μM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of ab271359—incubated 45 minutes at 30 °C. Followed by addition of 25 μL of luminescent reagent—incubated 45 minutes at room temperature. Followed by addition of 50 µl of proprietary detection reagent —incubated 45 minutes at room temperature. Luminescence was measured.
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精製度
= 90 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
45 kDa including tags -
領域
147 to 509 -
タグ
DDDDK tag N-Terminus , Avi tag C-Terminus
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標識
Biotin
特性
Our Abpromise guarantee covers the use of ab271359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 8 ng/µL DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Activin A receptor type IIA
- Activin receptor type 2A
- Activin receptor type IIA
see all -
機能
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
画像
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Functional Studies - (ab271359)
Kinase activity of ab271359 using casein as substrate.
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SDS-PAGE - (ab271359)SDS-PAGE analysis of ab271359.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271359 は論文での使用が確認できていません。
