Recombinant human Activin Receptor Type IA protein (Active) (ab271360)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human Activin Receptor Type IA protein (Active)
Activin Receptor Type IA タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 40 pmol/min/µgAssay Conditions: Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 μM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of Activin Receptor Type IA — incubated 45 minutes at 30 °C. Followed by addition of 25 μL of ADP-Glo reagent—incubated 45 minutes at room temperature. Followed by addition of 50 µl of Kinase Detection Reagent—incubated 45 minutes at room temperature. Luminescence was measured. -
精製度
>= 90 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
RKFKRRNQER LNPRDVEYGT IEGLITTNVG DSTLADLLDH SCTSGSGSGL PFLVQRTVAR QITLLECVGK GRYGEVWRGS WQGENVAVKI FSSRDEKSWF RETELYNTVM LRHENILGFI ASDMTSRHSS TQLWLITHYH EMGSLYDYLQ LTTLDTVSCL RIVLSIASGL AHLHIEIFGT QGKPAIAHRD LKSKNILVKK NGQCCIADLG LAVMHSQSTN QLDVGNNPRV GTKRYMAPEV LDETIQVDCF DSYKRVDIWA FGLVLWEVAR RMVSNGIVED YKPPFYDVVP NDPSFEDMRK VVCVDQQRPN IPNRWFSDPT LTSLAKLMKE CWYQNPSARL TALRIKKTLT KIDNSLDKLK TDC -
予測される分子量
67 kDa including tags -
領域
147 to 509 -
タグ
GST tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab271360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Activin A receptor type I
- Activin A receptor type II like kinase 2
- Activin receptor type I
see all -
機能
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis. -
組織特異性
Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. -
関連疾患
Defects in ACVR1 are a cause of fibrodysplasia ossificans progressiva (FOP) [MIM:135100]. FOP is a rare autosomal dominant disorder of skeletal malformations and progressive extraskeletal ossification. Heterotopic ossification in FOP begins in childhood and can be induced by trauma or may occur without warning. Bone formation is episodic and progressive, leading to extra-articular ankylosis of all major joints of the axial and appendicular skeleton, rendering movement impossible. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271360 は論文での使用が確認できていません。