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  1. Link

    recombinant-human-actin-regulatory-protein-capgmcp-ab95385.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Recombinant Human Actin Regulatory Protein CAPG/MCP (ab95385)

  • Datasheet
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Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

SDS-PAGE - Recombinant Human Actin Regulatory Protein CAPG/MCP (ab95385)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: MS, SDS-PAGE

    こちらの製品もご検討ください

     
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    ペプチド
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    関連製品

    製品の詳細

    • 製品名

      Recombinant Human Actin Regulatory Protein CAPG/MCP
      Actin Regulatory Protein CAPG/MCP タンパク質・ペプチド 製品一覧
    • 精製度

      > 95 % SDS-PAGE.
      ab95385 is purified using conventional chromatography techniques.
    • 発現系

      Escherichia coli
    • タンパク質長

      Full length protein
    • Animal free

      No
    • 由来

      Recombinant
      • 生物種

        Human
      • 配列

        MYTAIPQSGS PFPGSVQDPG LHVWRVEKLK PVPVAQENQG VFFSGDSYLV LHNGPEEVSH LHLWIGQQSS RDEQGACAVL AVHLNTLLGE RPVQHREVQG NESDLFMSYF PRGLKYQEGG VESAFHKTST GAPAAIKKLY QVKGKKNIRA TERALNWDSF NTGDCFILDL GQNIFAWCGG KSNILERNKA RDLALAIRDS ERQGKAQVEI VTDGEEPAEM IQVLGPKPAL KEGNPEEDLT ADKANAQAAA LYKVSDATGQ MNLTKVADSS PFALELLISD DCFVLDNGLC GKIYIWKGRK ANEKERQAAL QVAEGFISRM QYAPNTQVEI LPQGRESPIF KQFFKDWK

    関連製品

    • Related Products

      • Anti-Actin Regulatory Protein CAPG/MCP antibody (ab90873)

    特性

    Our Abpromise guarantee covers the use of ab95385 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Mass Spectrometry

      SDS-PAGE

    • 製品の状態

      Liquid
    • 備考

       This product was previously labelled as Actin Regulatory Protein CAPG

       

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)

    関連情報

    • 別名

      • Actin capping protein GCAP39
      • Actin regulatory protein CAP G
      • Actin regulatory protein CAP-G
      • AFCP
      • Capg
      • CAPG_HUMAN
      • Capping actin protein gelsolin like
      • Capping protein (actin filament) gelsolin like
      • Capping protein gelsolin like
      • Epididymis secretory protein Li 66
      • gCap39
      • Gelsolin like capping protein
      • HEL S 66
      • Macrophage capping protein
      • Macrophage-capping protein
      • mbh1
      • MCP
      • Myc basic motif homolog 1
      see all
    • 機能

      Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
    • 組織特異性

      Macrophages and macrophage-like cells.
    • 配列類似性

      Belongs to the villin/gelsolin family.
      Contains 3 gelsolin-like repeats.
    • 翻訳後修飾

      The N-terminus is blocked.
    • 細胞内局在

      Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P40121 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Actin Regulatory Protein CAPG/MCP (ab95385)
      SDS-PAGE - Recombinant Human Actin Regulatory Protein CAPG/MCP (ab95385)
      15% SDS-PAGE analysis of 3µg ab95385.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (0)

    ab95385 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab95385 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Please see customers request below:

    If you could arrange to have a replacement of the same item sent (different batch) that would be great.

    Many thanks for your speedy reply!

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2012

    Answer

    Thank you for kindly confirming these details. I am sorry this vial of protein has not worked for this customer.

    As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab95385 has been added to your order number PO-15699 - DG. This has Abcam order reference 1175608. This has a different lot number.

    I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vial, or if you have any further questions, please do not hesitate to let me know.

    Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Oct 11 2012

    Question

    Sorry for the delay, please see customers response below:

    Finally got the blot image from my student:

    Here are the details in regards to the experimental protocol:
    1. 30ng protein was loaded for each sample.

    2. Both samples (old and new batch of CapG) were run on the same blot (ie. same condition)

    3. As per all blots, the primary antibody used was rabbit anti-human CapG (GeneTex), secondary antibody anti-rabbit Ig (HRP-conjugated) (Millipore).

    4. Blots were imaged using IQ350, 10 minute exposure with ECL.

    New Old

    (GR20513-1) (GR20513-2)

    I would be grateful if you could seek clarification of this from the manufacturers.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2012

    Answer

    Thank you for your message and for kindly providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or credit note in compensation.

    I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Oct 10 2012

    Question

    Dear Supplier,

    Please see customers response below and attached regarding technical issue with ab95385- Actin Regulatory Protein CAPG (Human):

    We are having problem with the Abcam product. AB95385- Actin Regulatory Protein CAPG (Human) (100 microg)*

    Previous batches we have purchased- batch GR20513-2 and GR20513-3- produced a strong band on Western Blot (30ng loaded). The new batch that arrived GR20513-1- produces a barely visible band by Western in comparison to other loaded batches.

    If you would be able to look into this with the supplier that would be great.

    Please let me know if you require any further information.

    Looking forward for your response.


    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 20 2012

    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    I would like to reassure you that ab95385 Actin Regulatory Protein CAPG (Human)is tested and covered by our 6 month guarantee for use inSDS gels. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

    Reviewing this case, I would like to investigate this further for you and obtain some further information for quality monitoring. In order to do so, I would also appreciate if you can confirm some further details:

    1. Please provide information on the primary and secondary antibodies that has been used to detect this protein in western blot. Were these the samethat were used to detectthe first successful 2 batches of protein? Are the current vials of antibodies working well with other samples?

    2. I can recommend to run an SDS gel andassess the quality of the protein sample using coomassie blue, to ensure it has not degraded. Could you confirm if this has been tried already? What were the results?

    3. I would appreciate if you are able to provide any images, which would help us to assess the results (from the batches that have worked, and the ones that have not).

    I hope this information is helpful, thank you for your cooperation.Please do not hesitate to contact me again with the further requested details and I hope we can resolve this case for you as soonas possible.

    Read More

    Abcam Scientific Support

    Answered on Aug 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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