Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) (ab271358)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) -
生理活性
Specific Activity: ≥20 pmoles/min/µgAssay Conditions: 25 μl Acetyl Coenzyme A Carboxylase beta reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected. -
精製度
>= 80 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
277 kDa including tags -
タグ
His tag C-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab271358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.68% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- ACACB
- ACACB_HUMAN
- ACC-beta
see all -
機能
Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (By similarity). May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA-dependent inhibition of carnitine palmitoyltransferase 1. -
組織特異性
Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716). Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759). -
パスウェイ
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
配列類似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
翻訳後修飾
Phosphorylated by AMPK, leading to inactivation of the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis. -
細胞内局在
Mitochondrion. Nucleus. Endomembrane system. May associate with membranes. - Information by UniProt
画像
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Functional Studies - Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) (ab271358)Specific activity of ab271358.
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SDS-PAGE - Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) (ab271358)SDS-PAGE analysis of ab271358.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271358 は論文での使用が確認できていません。