Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品名Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active)
生理活性Specific Activity: ≥20 pmoles/min/µgAssay Conditions: 25 μl Acetyl Coenzyme A Carboxylase beta reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected.
精製度>= 80 % SDS-PAGE.
発現系Baculovirus infected Sf9 cells
タンパク質長Full length protein
予測される分子量277 kDa including tags
タグHis tag C-Terminus
Our Abpromise guarantee covers the use of ab271358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 0.68% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
機能Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (By similarity). May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA-dependent inhibition of carnitine palmitoyltransferase 1.
組織特異性Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716). Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759).
パスウェイLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
配列類似性Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
翻訳後修飾Phosphorylated by AMPK, leading to inactivation of the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis.
細胞内局在Mitochondrion. Nucleus. Endomembrane system. May associate with membranes.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.