Recombinant human AACT protein (ab182695)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human AACT protein
AACT タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2.
The IC50 value is <6 nM.
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
CHPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKN VIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHL LRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQD SAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFD PQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNA SALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDY N LNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGT EASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNP KQA -
予測される分子量
23 kDa including tags -
領域
23 to 423 -
タグ
His tag C-Terminus -
配列の追加情報
Mature form. AAH03559.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab182695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C. Reconstitute for long term storage.
pH: 7.4
Constituents: 0.88% Sodium chloride, 0.48% HEPES, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
関連情報
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別名
- SERPINA3
- AACT
- AACT_HUMAN
see all -
機能
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. -
組織特異性
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. -
関連疾患
Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. -
配列類似性
Belongs to the serpin family. -
ドメイン
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
細胞内局在
Secreted. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab182695 は論文での使用が確認できていません。