Recombinant HIV tat (mutated C22) protein (ab83352)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Blocking, Functional Studies, WB, SDS-PAGE
製品の詳細
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製品名
Recombinant HIV tat (mutated C22) protein
HIV tat タンパク質・ペプチド 製品一覧 -
生理活性
Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum. -
精製度
> 90 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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修飾
mutated C22
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab83352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Blocking
Functional Studies
Western blot
SDS-PAGE
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製品の状態
Lyophilized -
備考
Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C.
pH: 7.50
Constituents: 0.24% Monobasic dihydrogen sodium phosphate, 0.91% Mannitol, 0.284% Dibasic monohydrogen sodium phosphate, 0.328% Sodium phosphate, 0.0154% DTT, 2.5% Glycerol, 6.96% Sodium chloride -
再構成The protein should be reconstituted in apirogenic and sterile dH2O. The reconstituted solution has to be used immediately, since it is not stable in liquid form.
関連情報
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別名
- p14
- Tat
- Tat protein
- Transactivating regulatory protein
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関連性
The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab83352 は 1 報の論文で使用されています。
- El-Amine R et al. HIV-1 Tat protein induces DNA damage in human peripheral blood B-lymphocytes via mitochondrial ROS production. Redox Biol 15:97-108 (2018). PubMed: 29220699