製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Pichia pastoris

特性

Our Abpromise guarantee covers the use of ab91276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    ELISA

    SDS-PAGE

  • 精製度
    > 95 % SDS-PAGE.
    ab91276 is purified by proprietary chromatographic techniques. Purity is >95% by SDS-PAGE and RP-HPLC.
  • 製品の状態
    Liquid
  • 備考


    ab91276 contains the Hepatitis B Virus Surface Antigen immunodominant region.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at 4°C or -20°C. Avoid freeze / thaw cycle.

    pH: 9.30
    Constituents: 0.58% Sodium chloride, 15% Glycerol, 0.09% EDTA, 0.21% Sodium carbonate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • HBsAg
    • S gene subtype adw2
    • sAg
    • ssAg
  • 関連性
    Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • 細胞内局在
    Membrane Virion

参考文献

ab91276 has not yet been referenced specifically in any publications.

レビューと Q&A

1-7 of 7 Abreviews or Q&A

Answer

Thank you for contacting us.

This product is not tesed for endotoxins.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

We have not conducted any research experiements with this recombinant protein beyond noting that ab91276 is immunoreactive with sera of Hepatitis B virus-infected individuals.

The 226 amino acid protein is based on the sequence at the following link: http://www.uniprot.org/uniprot/Q773S4.

I hope this is helpful. Please contact us again if you have any further questions.

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Answer

Thank you for contacting Abcam.

We have a number of antibodies against Hepatitis B Surface Antigen and I have placed links to them below:

https://www.abcam.com/Hepatitis-B-Surface-Antigen-antibody-1044329-ab128198.html
https://www.abcam.com/Hepatitis-B-Surface-Antigen-antibody-B514M-ab110826.html
https://www.abcam.com/Hepatitis-B-Surface-Antigen-ayw-antibody-B1264M-ab110600.html
https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-S14-ab8636.html



If there is anything else I can help you with, please let me know.

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Question

Dear Technical Help,

The item in question is not an antibody, but an antigen (ab91276).
Below are the answers to your questionnaire:

Order Details
Antibody code: ab91276 (Antigen)
Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background
Lot number: GR55751-1

General Information
Antibody storage conditions (temperature/reconstitution etc): -20'C in aliquots
Description of the problem (high background, wrong band size, more bands, no band etc.): Multiple band sizes on WB
Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.): 0.2ug of ab91276
Sample preparation (Buffer/Protease inhibitors/Heating sample etc.): Invitrogen NuPAGE LDS sample buffer with NuPAGE reducing agent
Amount of protein loaded: 0.2ug
Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.): Reducing, Biorad Mini protean TGX AnykD precast gel.
Transfer and blocking conditions (Buffer/time period, Blocking agent etc.): Biorad TransBlot Turbo system, TransBlot Turbo Mini PVDF Transfer Packs, blocked using Invitrogen WesternBreeze blocking solution.
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step): Either anti-S, anti-PreS1 antibody or combination of both in Invitrogen WesternBreeze Antibody diluent incubation for 1h. Antibodies are gifts from other research groups. Wash using Invitrogen WesternBreeze Antibody wash - 4x 5min washes.
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step): goat anti-mouse HRP (AbD Serotec), in Invitrogen WesternBreeze Antibody diluent incubation for 30min. Wash using Invitrogen WesternBreeze Antibody wash - 4x 5min washes.
Detection method (ECL, ECLPlus etc.): ECLplus, ECL prime or Biorad ImmunStar WesternC Kit.
Positive and negative controls used (please specify): Antigen ab91276 is meant to be the positive control
Optimization attempts (problem solving)
How many times have you tried the Western? 2
Have you run a "No Primary" control? No
Do you obtain the same results every time? Yes
e.g. are the background bands always in the same place?
What steps have you altered? Reduced antigen concentration
Image: Please see attached, PVDF membrane probed with combination of anti-S and anti-PreS1 antibody. Left lane is Invitrogen MagicMark XP protein standard (band sizes from bottom up: 20kDa, 30kDa, 40kDa, 50kDa, 60kDa). Right lane is 0.2ug ab91276, dark bands seen at 39kDa and 24kDa (which are the expected sizes of L and S proteins respectively). Weaker bands seen at 55kDa, ˜33kDa (expected size of M protein) 18kDa and 10kDa.
Additional notes:
The reason for my question regarding the production of the antigen is that your datasheet states 'Recombinant full length Hepatitis B Surface Antigen (Adw) expressed in P. pastoris". Hepatitis B virus has 3 surface proteins (L, M and S) and when I run a WB, I obtain a band at the expected size of the L protein when I use anti-PreS1 antibody on it's own or combined with anti-S antibody, as well as bands at expected sizes of M and S when using anti-S antibody. My question is whether the expression vector used to express the antigen actually contains both the PreS1 and PreS2 domains - this would result in expression in P. pastoris of all 3 surface proteins.
Regards

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Answer

Thank you for taking the time to complete the questionnaire and send an image. This has been helpful to our quality investigations.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there very few tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial of protein.

In this case, I am pleased to offer you a free of charge replacement or credit note in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I have discussed this with our testing source and can confrim that this antibody should only detect the S protein at 24kDa.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB. In the event that a product is not functioning in the tested applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.

Order Details
Antibody code:
Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number
Purchase order number
or preferably Abcam order number:
General Information
Antibody storage conditions (temperature/reconstitution etc)
Description of the problem (high background, wrong band size, more bands, no band etc.)
Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
Amount of protein loaded
Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Detection method (ECL, ECLPlus etc.)
Positive and negative controls used (please specify)
Optimization attempts (problem solving)
How many times have you tried the Western?
Have you run a "No Primary" control?
Yes No
Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?
What steps have you altered?
Additional Notes:
Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

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Answer

Merci de nous avoir contactés.

ab67704 : tag GST, 40 kDa
ab69741 : pas de tag, 57.5 kDa
ab69748 : pas de tag, 64.5 kDa
ab69749 : pas de tag, 70 kDa
ab91276 : pas de tag, 24 kDa

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Application
Other

Review text: Used as a standardised positive control for an in-house HBsAg ELISA as well as commercial ELISA. Used at concentrations ranging from 10ng to 0.125ng - all concentrations gave positive results on ELISAs.
Sample: Human Serum

Primary antibody (in addition to 'Hepatitis B Surface Antigen (Adw) protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

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Verified customer

投稿 Dec 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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