Recombinant dog PDE3A protein (Active) (ab271677)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant dog PDE3A protein (Active)
PDE3A タンパク質・ペプチド 製品一覧 -
生理活性
Assay conditions:10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, 0.02% TritonX, and serial dilutions of PDE3A at 37ºC for 20 mins. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Absorbance was measured at 620 nm. -
精製度
>= 70 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Dog -
予測される分子量
75 kDa -
領域
343 to 998 -
タグ
DDDDK tag C-Terminus -
配列の追加情報
Genbank: XP_005637137.
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特性
Our Abpromise guarantee covers the use of ab271677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 5''-cyclic phosphodiesterase A
- cAMP phosphodiesterase myocardial cGMP inhibited
- CGI PDE
see all -
機能
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily. -
細胞内局在
Membrane. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271677 は論文での使用が確認できていません。