• 製品名

  • 発現系

    Escherichia coli
  • アクセッション番号

  • タンパク質長

    Full length protein
  • Animal free

  • 由来

    • 生物種

    • 配列

    • 予測される分子量

      25 kDa including tags
    • 領域

      25 to 244
    • タグ

      His tag C-Terminus


Our Abpromise guarantee covers the use of ab753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション



  • 製品の状態

  • Concentration information loading...


  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 4.00
    Constituent: 0.082% Sodium acetate


  • 別名

    • Alternative prion protein; major prion protein
    • AltPrP
    • ASCR
    • CD230
    • CD230 antigen
    • CJD
    • GSS
    • KURU
    • Major prion protein
    • p27 30
    • Prion protein
    • Prion related protein
    • PRIP
    • PRNP
    • PrP
    • PrP27 30
    • PrP27-30
    • PrP33-35C
    • PrPC
    • PrPSc
    • Sinc
    see all
  • 機能

    The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis (By similarity). Isoform 2 may act as a growth suppressor by arresting the cell cycle at the G0/G1 phase. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).
  • 関連疾患

    Note=PrP is found in high quantity in the brain of humans and animals infected with neurodegenerative diseases known as transmissible spongiform encephalopathies or prion diseases, like: Creutzfeldt-Jakob disease (CJD), fatal familial insomnia (FFI), Gerstmann-Straussler disease (GSD), Huntington disease-like type 1 (HDL1) and kuru in humans; scrapie in sheep and goat; bovine spongiform encephalopathy (BSE) in cattle; transmissible mink encephalopathy (TME); chronic wasting disease (CWD) of mule deer and elk; feline spongiform encephalopathy (FSE) in cats and exotic ungulate encephalopathy (EUE) in nyala and greater kudu. The prion diseases illustrate three manifestations of CNS degeneration: (1) infectious (2) sporadic and (3) dominantly inherited forms. TME, CWD, BSE, FSE, EUE are all thought to occur after consumption of prion-infected foodstuffs.
    Defects in PRNP are the cause of Creutzfeldt-Jakob disease (CJD) [MIM:123400]. CJD occurs primarily as a sporadic disorder (1 per million), while 10-15% are familial. Accidental transmission of CJD to humans appears to be iatrogenic (contaminated human growth hormone (HGH), corneal transplantation, electroencephalographic electrode implantation, etc.). Epidemiologic studies have failed to implicate the ingestion of infected annimal meat in the pathogenesis of CJD in human. The triad of microscopic features that characterize the prion diseases consists of (1) spongiform degeneration of neurons, (2) severe astrocytic gliosis that often appears to be out of proportion to the degree of nerve cell loss, and (3) amyloid plaque formation. CJD is characterized by progressive dementia and myoclonic seizures, affecting adults in mid-life. Some patients present sleep disorders, abnormalities of high cortical function, cerebellar and corticospinal disturbances. The disease ends in death after a 3-12 months illness.
    Defects in PRNP are the cause of fatal familial insomnia (FFI) [MIM:600072]. FFI is an autosomal dominant disorder and is characterized by neuronal degeneration limited to selected thalamic nuclei and progressive insomnia.
    Defects in PRNP are the cause of Gerstmann-Straussler disease (GSD) [MIM:137440]. GSD is a heterogeneous disorder and was defined as a spinocerebellar ataxia with dementia and plaquelike deposits. GSD incidence is less than 2 per 100 million live births.
    Defects in PRNP are the cause of Huntington disease-like type 1 (HDL1) [MIM:603218]. HDL1 is an autosomal dominant, early onset neurodegenerative disorder with prominent psychiatric features.
    Defects in PRNP are the cause of kuru (KURU) [MIM:245300]. Kuru is transmitted during ritualistic cannibalism, among natives of the New Guinea highlands. Patients exhibit various movement disorders like cerebellar abnormalities, rigidity of the limbs, and clonus. Emotional lability is present, and dementia is conspicuously absent. Death usually occurs from 3 to 12 month after onset.
    Defects in PRNP are the cause of spongiform encephalopathy with neuropsychiatric features (SENF) [MIM:606688]; an autosomal dominant presenile dementia with a rapidly progressive and protracted clinical course. The dementia was characterized clinically by frontotemporal features, including early personality changes. Some patients had memory loss, several showed aggressiveness, hyperorality and verbal stereotypy, others had parkinsonian symptoms.
  • 配列類似性

    Belongs to the prion family.
  • ドメイン

    The normal, monomeric form has a mainly alpha-helical structure. The disease-associated, protease-resistant form forms amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Disease mutations may favor intermolecular contacts via short beta strands, and may thereby trigger oligomerization.
    Contains an N-terminal region composed of octamer repeats. At low copper concentrations, the sidechains of His residues from three or four repeats contribute to the binding of a single copper ion. Alternatively, a copper ion can be bound by interaction with the sidechain and backbone amide nitrogen of a single His residue. The observed copper binding stoichiometry suggests that two repeat regions cooperate to stabilize the binding of a single copper ion. At higher copper concentrations, each octamer can bind one copper ion by interactions with the His sidechain and Gly backbone atoms. A mixture of binding types may occur, especially in the case of octamer repeat expansion. Copper binding may stabilize the conformation of this region and may promote oligomerization.
  • 翻訳後修飾

    The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion.
    Isoform 2 is sumoylated by SUMO1.
  • 細胞内局在

    Cell membrane. Golgi apparatus and Cytoplasm. Nucleus. Accumulates outside the secretory route in the cytoplasm, from where it relocates to the nucleus.
  • Information by UniProt


  • SDS-PAGE of 2 µg PrPc (lane 1).


This product has been referenced in:

  • Legleiter LR  et al. Copper deficiency in the young bovine results in dramatic decreases in brain copper concentration but does not alter brain prion protein biology. J Anim Sci 86:3069-78 (2008). WB . Read more (PubMed: 18599661) »
  • Legleiter LR  et al. Decreased brain copper due to copper deficiency has no effect on bovine prion proteins. Biochem Biophys Res Commun 352:884-8 (2007). Read more (PubMed: 17157816) »
See all 3 Publications for this product

レビューと Q&A

1-9 of 9 Abreviews or Q&A


Ab753: is derived from cattle
Ab74200: is derived from sheep ARR genotype
Ab90464: is derived from sheep
Ab90482: is derived from hamster
Ab90483: is derived from hamster
ab140567: is a human prion protein
Thus, it is ab140567 that would be suitable for you.
I will also make sure that we update our datasheets with this crucial information.

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Thank you for your enquiry.

I can confirm thatto our knowledge Prion protein PrP ab753 is not infectious. It is a recombinant prion protein HIS tagged, not glycosylated and the product will be completely digested by Proteinase K.

As far as I am aware, it is the mutated forms of the prion protein that are infectious, such as cPrP.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Thanks for the additional information. Here is a link to an HRP-conjugated secondary antibody you could for western blotting with ab703: https://www.abcam.com/Rabbit-IgG-secondary-antibody-ab16284.html I hope this is helpful. Please contact me again if  you have any further questions.

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Thank you for your enquiry. I would be happy to help you find a secondary antibody for use with ab703. If you could let me know what application you would use the antibody for and what conjugate you would like attached to the secondary (eg. HRP, biotin, what color fluorophore) then I can recommend a product.

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Thank you for contacting us. The immunogen used for ab52604 was a smaller peptide from human Prion protein, whereas the protein ab753 is recombinant bovine Prion protein. Ab52604 was not tested for bovine and is not guaranteed to react; a better choice for use with ab753 is ab703, which used ab753 as the immunogen and will definately detect it. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Thank you for your enquiry. Ab753 is a protein solution in 10 mM sodium acetate buffer; it used to come in lyophilized form but now comes reconstituted in 10 mM sodium acetate buffer. Ab1391 is a protein solution in 50 mM sodium acetate buffer. This is all the information that the originator was able to give me. If you have any more questions, please contact us again.

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The antibody does not require any special handling facilities.

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His tag is at the carboxy terminus of the recombinant bovine PrP.

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