Application
Western blot
Used the protein as a positive control in Western Blot of a GFP-tagged recombinant protein preparation. Result: good specific signal, no nonspecific bands detected.
Block: TBS + 3 % milk 30 min.
Antibody incubation: HRP-conjucated goat polyclonal to GFP (Abcam ab6663) in 1:2000 dilution, 60 min.
Wash: 4 x 5 min TBS-T.
Detection: 5 min incubation in TMB Liquid Substrate System (Sigma).
Block: TBS + 3 % milk 30 min.
Antibody incubation: HRP-conjucated goat polyclonal to GFP (Abcam ab6663) in 1:2000 dilution, 60 min.
Wash: 4 x 5 min TBS-T.
Detection: 5 min incubation in TMB Liquid Substrate System (Sigma).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 08 2014