Anti-REC8 抗体 [EPR16189] (ab192241)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16189] to REC8
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-REC8 antibody [EPR16189]
REC8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16189] to REC8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human, mouse and rat testis and Jurkat lysates; Human, mouse and rat testis tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16189 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab192241の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 67 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 67 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. -
組織特異性
Expressed in testis and thymus. -
配列類似性
Belongs to the rad21 family. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Nucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. - Information by UniProt
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参照データベース
- Entrez Gene: 9985 Human
- Entrez Gene: 56739 Mouse
- Entrez Gene: 290227 Rat
- Omim: 608193 Human
- SwissProt: O95072 Human
- SwissProt: Q8C5S7 Mouse
- SwissProt: Q6AYJ4 Rat
- Unigene: 419259 Human
see all -
別名
- Cohesin Rec8p antibody
- Human homolog of rad21 S. pombe antibody
- Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-REC8 antibody [EPR16189] (ab192241) at 1/10000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 67 kDa -
All lanes : Anti-REC8 antibody [EPR16189] (ab192241) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 67 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (10)
ab192241 は 10 報の論文で使用されています。
- Choi EH et al. Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes. Genome Biol 23:70 (2022). PubMed: 35241136
- Koh YE et al. The Kleisin Subunits of Cohesin are Involved in the Fate Determination of Embryonic Stem Cells. Mol Cells 45:820-832 (2022). PubMed: 36172976
- Chatzidaki EE et al. Ovulation suppression protects against chromosomal abnormalities in mouse eggs at advanced maternal age. Curr Biol 31:4038-4051.e7 (2021). PubMed: 34314679
- Hsieh CL et al. MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA. EMBO J 39:e103614 (2020). PubMed: 32677148
- Li Y et al. Cyclin B3 is required for metaphase to anaphase transition in oocyte meiosis I. J Cell Biol 218:1553-1563 (2019). PubMed: 30770433
- Zhao J et al. REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells. Oncol Rep 39:1583-1590 (2018). PubMed: 29393474
- Castro W et al. The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity. Nat Immunol 19:809-820 (2018). PubMed: 29967452
- Mu X et al. Retinoic acid-induced CYP51 nuclear translocation promotes meiosis prophase I process and is correlated to the expression of REC8 and STAG3 in mice. Biol Open 7:N/A (2018). PubMed: 30420384
- Szydłowska A et al. Manipulating Cohesin Levels in Live Mouse Oocytes. Methods Mol Biol 1818:113-128 (2018). PubMed: 29961260
- Suzuki A et al. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. Nat Commun 7:11056 (2016). PubMed: 27025988