DyLight® Fluorochrome conjugated secondary antibodies

An alternative to conventional dyes.

DyLight® family of dyes

DyLight® fluorochromes exhibit high fluorescence intensity, photostability and water solubility and remain fluorescent from pH 4.0 and pH 9.0. Additionally, the water solubility of the DyLight® fluorochromes allows a high dye-to-antibody ratio to be achieved without causing precipitation of conjugates.

DyLight® fluorochromes have absorption maxima ranging from 350 nm to 777 nm, covering the entire visible light spectrum. Both the absorption and emission properties of the DyLight® fluorochromes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation.

Our catalog includes secondary antibodies conjugated with DyLight® 488, DyLight​® 550, DyLight​® 594 and DyLight® 650.

Fig 1. Emission spectra of DyLight®​ fluorochromes available in our catalog. 

Antibody conjugate highlights

• Large range of different specificities
• ​Optimized for conjugate performance - affinity-purified or pre-adsorbed antibodies
• Consistent and stable - stable for 1 year at 4°C, providing consistent performance for the duration of use
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Advantages

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• Bright fluorescence - intense emission provides high sensitivity
• Narrow emission spectra - negligible bleed-through between fluorochrome channels makes multi-color imaging easy
• Highly photostable - better resistance to photobleaching
• ​Completely stable in buffers ranging from pH 4.0-9.0
• Instrument compatibility - DyLight® can be visualized with common laser and filter settings

DyLight® dye properties

DyLight® fluorochromes are suitable for fluorescence microscopy and flow cytometry.

Table 1. Spectral properties of DyLight®​ fluorochromes. 

ε: Molar extinction coefficient at the absorption maximum

For further information or help, contact our Scientific Support team.

​DyLight®​ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Cy®​ and CyDye® are trademarks of Cytiva.