Anti-RBX1 抗体 [EPR6850(B)] (ab133565)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6850(B)] to RBX1
- Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RBX1 antibody [EPR6850(B)]
RBX1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6850(B)] to RBX1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IP: Jurkat whole cell lysate. Flow Cyt: Jurkat cells. ICC/IF: HeLa cells. IHC-P: Rat and mouse breast tissue. Human lung carcinoma tissue. WB: Jurkat whole cell lysate. Mouse and rat heart lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 2.18 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6850(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133565の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified format use at 1/100 to 1/1000 dilution |
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IP |
1/10 - 1/100.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified format use 1/100 to 1/250 dilution |
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WB |
1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
For unpurified format use 1/1000 to 1/10000 dilution |
|
ICC/IF | (1) |
1/50.
|
特記事項 |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified format use at 1/100 to 1/1000 dilution |
IP
1/10 - 1/100. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified format use 1/100 to 1/250 dilution |
WB
1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa). For unpurified format use 1/1000 to 1/10000 dilution |
ICC/IF
1/50. |
ターゲット情報
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機能
E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. -
組織特異性
Widely expressed. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the RING-box family.
Contains 1 RING-type zinc finger. -
ドメイン
The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9978 Human
- Entrez Gene: 56438 Mouse
- Entrez Gene: 300084 Rat
- Omim: 603814 Human
- SwissProt: P62877 Human
- SwissProt: P62878 Mouse
- Unigene: 474949 Human
- Unigene: 29405 Mouse
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別名
- BA554C12.1 antibody
- E3 ubiquitin-protein ligase RBX1 antibody
- FLJ60363 antibody
see all
画像
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All lanes : Anti-RBX1 antibody [EPR6850(B)] (ab133565) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Image produced using the purified version.
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Purified ab133565 at 1:20 dilution (1µg) immunoprecipitating RBX1 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg.
Lane 2 (+): ab133565 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133565 in Jurkat whole cell lysate.VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 13 kDa -
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling RBX1 with Purified ab133565 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RBX1 with Purified ab133565 at 1:50 dilution (3.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat breast tissue sections labeling RBX1 with Purified ab133565 at 1:2000 dilution (0.099 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling RBX1 with Purified ab133565 at 1:2000 dilution (0.099 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling RBX1 with Purified ab133565 at 1:2000 dilution (0.099 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab133565 は 13 報の論文で使用されています。
- Gupta S et al. Functional Characterization of Non-Ankyrin Repeat Domains of Orientia tsutsugamushi Ank Effectors Reveals Their Importance for Molecular Pathogenesis. Infect Immun 90:e0062821 (2022). PubMed: 35435726
- Zhang Q et al. Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest. Brain Sci 12:N/A (2022). PubMed: 35884735
- Wu Q et al. ROC1 promotes the malignant progression of bladder cancer by regulating p-I?Ba/NF-?B signaling. J Exp Clin Cancer Res 40:158 (2021). PubMed: 33962660
- Adcox HE et al. Orientia tsutsugamushi Nucleomodulin Ank13 Exploits the RaDAR Nuclear Import Pathway To Modulate Host Cell Transcription. mBio 12:e0181621 (2021). PubMed: 34340535
- Lanzafame M et al. Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation. Nucleic Acids Res 49:10911-10930 (2021). PubMed: 34581821