Anti-RBP1 抗体 (ab31106)
Key features and details
- Goat polyclonal to RBP1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RBP1 antibody
RBP1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RBP1 -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow -
免疫原
Synthetic peptide:
VEGVVCKQVFKKVQ
, corresponding to C terminal amino acids 121-134 of Human PRBPI -
ポジティブ・コントロール
- NIH 3T3 lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Ab31106 underwent ammonium sulphate precipitation prior to antigen affinity chromatography purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31106の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
---|
ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Intracellular transport of retinol. -
組織特異性
Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. -
配列類似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
ドメイン
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 537379 Cow
- Entrez Gene: 5947 Human
- Entrez Gene: 19659 Mouse
- Omim: 180260 Human
- SwissProt: P02694 Cow
- SwissProt: P09455 Human
- SwissProt: Q00915 Mouse
- Unigene: 529571 Human
see all -
別名
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
see all
画像
-
Lane 1 : Anti-RBP1 antibody (ab31106) at 2 µg/ml
Lane 2 : Anti-RBP1 antibody (ab31106) at 0.5 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : U251 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 15 kDa -
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr with ab31106 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
-
Anti-RBP1 antibody (ab31106) at 0.1 µg/ml + NIH 3T3 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab31106 は 2 報の論文で使用されています。
- Ludvigsen M et al. Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer. Int J Mol Sci 21:N/A (2020). PubMed: 32422974
- Zhu H et al. Proteomic analysis of testis biopsies in men treated with transient scrotal hyperthermia reveals the potential targets for contraceptive development. Proteomics 10:3480-93 (2010). WB ; Human . PubMed: 20815088