Anti-RBP1 抗体 (ab104452)
Key features and details
- Goat polyclonal to RBP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RBP1 antibody
RBP1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RBP1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
VEGVVCKQVFKKVQ
conjugated to KLH, corresponding to C terminal amino acids 122-135 of Human RBP1. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104452の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 16 kDa.
|
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| IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 16 kDa. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Intracellular transport of retinol. -
組織特異性
Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. -
配列類似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
ドメイン
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5947 Human
- Entrez Gene: 19659 Mouse
- Omim: 180260 Human
- SwissProt: P09455 Human
- SwissProt: Q00915 Mouse
- Unigene: 529571 Human
- Unigene: 279741 Mouse
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別名
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (ab104452)ab104452, at 5 µg/ml, staining RBP1 in formalin-fixed, paraffin-embedded Human Liver by Immunohistochemistry, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (ab104452)ab104452, at 5 µg/ml, staining RBP1 in formalin-fixed, paraffin-embedded Human Pancreas by Immunohistochemistry, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Western blot - Anti-RBP1 antibody (ab104452)Anti-RBP1 antibody (ab104452) at 0.1 µg/ml + NIH 3T3 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 16 kDa
Primary incubation was 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (ab104452)This image is courtesy of an Abreview submitted by Carl Hobbsab104452 staining mouse liver sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab104452 at 1/100 in TBS/BSA/azide for 2h at 21°C. A biotinylated rabbit anti-goat polyclonal antibody at 1/250 was used as the secondary antibody. Red arrowheads indicate strongly stained Kupffer cells.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab104452 は論文での使用が確認できていません。
