Anti-Rbm15/OTT 抗体 (ab244374)
Key features and details
- Rabbit polyclonal to Rbm15/OTT
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Rbm15/OTT antibody
Rbm15/OTT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rbm15/OTT -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Rbm15/OTT aa 250-400.
Database link: Q96T37 -
ポジティブ・コントロール
- IHC-P: Human colon tissue. WB: K562, NIH/3T3 and NBT-II cell lysate. ICC/IF: U-2 OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab244374の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be implicated in HOX gene regulation. -
関連疾患
A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene. -
配列類似性
Belongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains.
Contains 1 SPOC domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 64783 Human
- Entrez Gene: 229700 Mouse
- Entrez Gene: 684233 Rat
- Omim: 606077 Human
- SwissProt: Q96T37 Human
- SwissProt: Q6PHZ5 Mouse
- Unigene: 435947 Human
- Unigene: 708172 Human
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別名
- One twenty two protein antibody
- One-twenty two protein 1 antibody
- OTT antibody
see all
画像
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Rbm15/OTT (green) using ab244374 at 4 µg/ml in ICC/IF.
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Formalin-fixed, paraffin-embedded human lymph node tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human testis tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
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Anti-Rbm15/OTT antibody (ab244374) at 0.4 µg/ml + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
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All lanes : Anti-Rbm15/OTT antibody (ab244374) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab244374 は 4 報の論文で使用されています。
- Yang F et al. Circ-CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with RBM15. Cell Prolif 56:e13344 (2023). PubMed: 36181462
- Wang X et al. RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent. J Exp Clin Cancer Res 40:80 (2021). PubMed: 33637103
- Zhang J et al. To Develop and Validate the Combination of RNA Methylation Regulators for the Prognosis of Patients with Gastric Cancer. Onco Targets Ther 13:10785-10795 (2020). PubMed: 33122917
- Huang B et al. Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model. Front Endocrinol (Lausanne) 10:415 (2019). PubMed: 31316467