Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ES cells)
Specification
Mouse ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used:
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: Doublet at 55KDa kDa
Positive control
Undifferentiated Embryonic Stem cells and Embryonic Stem cells differentiated for 8 days.
Additional data
Additional Notes
Lysates were prepared in NP40 buffer followed by gentle sonication. Protein concentrations were calculated using the Pierce assay and 20ug of each lysate was boiled in Laemlli buffer and loaded onto a 7.5% SDS PAG. Protein was transferred onto Hybond C+ and probed in 5% Milk.
This antibody behaves excellently and detects both p48 and an orthologue; running as a doublet.
This antibody behaves excellently and detects both p48 and an orthologue; running as a doublet.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 20 2007