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  1. Link

    rbbp4-antibody-ab1765.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-RBBP4 抗体 (ab1765)

  • Datasheet
Reviews (8)Q&A (1)References (13)

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Western blot - Anti-RBBP4 antibody (ab1765)
  • Western blot - Anti-RBBP4 antibody (ab1765)

Key features and details

  • Rabbit polyclonal to RBBP4
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human RBBP4 protein (ab130037)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-RBBP4 antibody
    RBBP4 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to RBBP4
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human RBBP4 aa 406-425.
    Sequence:

    CENIYNDEDPEGSVDPEGQGS

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Locus link: 350005 Alternative names: similar to chromatin assembly factor 1 p48 subunit


    A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • 一次抗体 備考

    A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • ISWI/NURF/CHRAC
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC2
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Histone chaperones
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human RBBP4 protein (ab130037)

アプリケーション

Our Abpromise guarantee covers the use of ab1765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47.6 kDa).

ターゲット情報

  • 機能

    Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • 配列類似性

    Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 6 WD repeats.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession Q09028 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 395658 Chicken
    • Entrez Gene: 5928 Human
    • Entrez Gene: 19646 Mouse
    • Omim: 602923 Human
    • SwissProt: Q9W7I5 Chicken
    • SwissProt: Q09028 Human
    • SwissProt: Q60972 Mouse
    • Unigene: 16003 Human
    • Unigene: 12145 Mouse
    see all
  • 別名

    • CAF I p48 antibody
    • CAF-1 subunit C antibody
    • CAF-I 48 kDa subunit antibody
    • CAF-I p48 antibody
    • Chromatin assembly factor 1 subunit C antibody
    • Chromatin assembly factor I p48 subunit antibody
    • Chromatin assembly factor/CAF 1 p48 subunit antibody
    • Histone-binding protein RBBP4 antibody
    • MSI1 protein homolog antibody
    • Nucleosome-remodeling factor subunit RBAP48 antibody
    • NURF55 antibody
    • RB binding protein 4 chromatin remodeling factor antibody
    • RbAp 48 antibody
    • RBAP48 antibody
    • RBBP-4 antibody
    • RBBP4 antibody
    • RBBP4_HUMAN antibody
    • Retinoblastoma binding protein 4 antibody
    • Retinoblastoma binding protein p48 antibody
    • Retinoblastoma-binding protein 4 antibody
    • Retinoblastoma-binding protein p48 antibody
    see all

画像

  • Western blot - Anti-RBBP4 antibody (ab1765)
    Western blot - Anti-RBBP4 antibody (ab1765)
    Lane 1 : Anti-RBBP4 antibody (ab1765) at 1/500 dilution
    Lane 2 : Anti-RBBP4 antibody (ab1765) at 1/1000 dilution

    All lanes : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Predicted band size: 47.6 kDa

  • Western blot - Anti-RBBP4 antibody (ab1765)
    Western blot - Anti-RBBP4 antibody (ab1765)
    Anti-RBBP4 antibody (ab1765) at 1/1000 dilution + Human LCL whole cell lysate

    Secondary
    Anti-rabbit IgG HRP

    Developed using the ECL technique.

    Predicted band size: 47.6 kDa
    Observed band size: 40,55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

プロトコール

  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (13)

    ab1765 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1765 は 13 報の論文で使用されています。

    • Li D  et al. Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nat Commun 9:2829 (2018). PubMed: 30026490
    • Jin X  et al. Overexpression of retinoblastoma-binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3-kinase/protein kinase B pathway suppression. Mol Med Rep 18:1571-1581 (2018). PubMed: 29901205
    • Cohen C  et al. Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis. PLoS Pathog 14:e1007313 (2018). PubMed: 30235352
    • Sun D  et al. LncRNA GAS5 inhibits microglial M2 polarization and exacerbates demyelination. EMBO Rep N/A:N/A (2017). PubMed: 28808113
    • Bode D  et al. Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells. Mol Cell Proteomics 15:878-91 (2016). WB . PubMed: 26714524
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-9 of 9 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RbAp48 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample
    Human Tissue sections (Human fibromatosis)
    Specification
    Human fibromatosis
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 05 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RbAp48 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Human Cell (293T)
    Specification
    293T
    Permeabilization
    Yes - Triton 1%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 02 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RbAp48 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Sample
    Dog Tissue sections (MDCK pellets in paraffin)
    Specification
    MDCK pellets in paraffin
    Permeabilization
    Yes - Tween-20
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 04 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RbAp48 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (HeLa cells)
    Specification
    HeLa cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X 100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Alexander Rapp

    Verified customer

    投稿 Apr 18 2008

    Immunoprecipitation abreview for Anti-RbAp48 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Human HeLa cell lysate)
    Specification
    Human HeLa cell lysate
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    投稿 May 15 2007

    Western blot abreview for Anti-RbAp48 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse ES cells)
    Specification
    Mouse ES cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 20 2007

    Question

    I was wondering if this antibody has been tested for IF yet. If not, would it be possible to obtain an aliquot to test for this purpose?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2006

    Answer

    This product has been tested in IF and would be gaurateed to work in that application.

    Read More

    Abcam Scientific Support

    Answered on Oct 12 2006

    Western blot abreview for Anti-RbAp48 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    25 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 12 2006

    Western blot abreview for Anti-RbAp48 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LCL)
    Specification
    LCL
    Blocking step
    Other as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Mr. Hannes Meyer

    Verified customer

    投稿 Dec 12 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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    • 細胞生物学
    • 発生
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    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
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