Anti-RBBP4 抗体 (ab1765)
Key features and details
- Rabbit polyclonal to RBBP4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RBBP4 antibody
RBBP4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RBBP4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chicken, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human RBBP4 aa 406-425.
Sequence:CENIYNDEDPEGSVDPEGQGS
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特記事項
Locus link: 350005 Alternative names: similar to chromatin assembly factor 1 p48 subunitA protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
一次抗体 備考
A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab1765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47.6 kDa). |
ターゲット情報
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機能
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. -
配列類似性
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 395658 Chicken
- Entrez Gene: 5928 Human
- Entrez Gene: 19646 Mouse
- Omim: 602923 Human
- SwissProt: Q9W7I5 Chicken
- SwissProt: Q09028 Human
- SwissProt: Q60972 Mouse
- Unigene: 16003 Human
see all -
別名
- CAF I p48 antibody
- CAF-1 subunit C antibody
- CAF-I 48 kDa subunit antibody
see all
画像
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Western blot - Anti-RBBP4 antibody (ab1765)Lane 1 : Anti-RBBP4 antibody (ab1765) at 1/500 dilution
Lane 2 : Anti-RBBP4 antibody (ab1765) at 1/1000 dilution
All lanes : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 47.6 kDa -
Western blot - Anti-RBBP4 antibody (ab1765)Anti-RBBP4 antibody (ab1765) at 1/1000 dilution + Human LCL whole cell lysate
Secondary
Anti-rabbit IgG HRP
Developed using the ECL technique.
Predicted band size: 47.6 kDa
Observed band size: 40,55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
プロトコール
データシートおよび資料
参考文献 (13)
ab1765 は 13 報の論文で使用されています。
- Li D et al. Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nat Commun 9:2829 (2018). PubMed: 30026490
- Jin X et al. Overexpression of retinoblastoma-binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3-kinase/protein kinase B pathway suppression. Mol Med Rep 18:1571-1581 (2018). PubMed: 29901205
- Cohen C et al. Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis. PLoS Pathog 14:e1007313 (2018). PubMed: 30235352
- Sun D et al. LncRNA GAS5 inhibits microglial M2 polarization and exacerbates demyelination. EMBO Rep N/A:N/A (2017). PubMed: 28808113
- Bode D et al. Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells. Mol Cell Proteomics 15:878-91 (2016). WB . PubMed: 26714524
