Anti-Rb2 p130 抗体 (ab6545)
Key features and details
- Rabbit polyclonal to Rb2 p130
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Rb2 p130 antibody
Rb2 p130 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rb2 p130 -
由来種
Rabbit -
特異性
No reaction is observed against other related tumor supressor proteins. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide (Human) conjugated to KLH.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
精製度
Whole antiserum -
特記事項（精製）
This product was prepared from monospecific antiserum by delipidation and defibrination. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab6545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/500 - 1/1000. |
ターゲット情報
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関連性
Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 5934 Human
- Entrez Gene: 19651 Mouse
- Entrez Gene: 81758 Rat
- Omim: 180203 Human
- SwissProt: Q08999 Human
- SwissProt: 235580 Mouse
- SwissProt: Q64700 Mouse
- SwissProt: O55081 Rat
see all -
別名
- 130 kDa retinoblastoma associated protein antibody
- FLJ26459 antibody
- p130 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (ab6545)Image from Lu Y et al., Dev Biol. 2007;310(2):442-53. Fig 7.; doi: 10.1016/j.ydbio.2007.08.007 with permission from Elsevierab6545 staining Rb2 p130 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde in PBS at 4°C overnight, embedded in paraffin was and section a 7μm. Endogenous enzyme activity was quenched by 2% H2O2 in methanol (30 minutes). Sections were blocked wirh 2% BSA, 5% serum and 0.1% Trition X-100 in PBS. Samples were incubated with primary antibody (1/500 in blocking buffer). White arrow heads mark epithelial cells expressing high levels of protein in nuclear and perinuclear locations.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 antibody (ab6545)Immunohistochemical staining of mouse tissue using anti-pRb2 / p130 antiserum.
The staining shows the location of pRb2 / p130 in developing mouse tissue.
プロトコール
参考文献 (3)
ab6545 は 3 報の論文で使用されています。
- Herriges MJ et al. Long noncoding RNAs are spatially correlated with transcription factors and regulate lung development. Genes Dev 28:1363-79 (2014). PubMed: 24939938
- Cerezo A et al. Absence of Caveolin-1 Increases Proliferation and Anchorage-Independent Growth by a Rac-Dependent, Erk-Independent Mechanism. Mol Cell Biol : (2009). WB ; Mouse . PubMed: 19620284
- Lu Y et al. Transgenic over-expression of the microRNA miR-17-92 cluster promotes proliferation and inhibits differentiation of lung epithelial progenitor cells. Dev Biol 310:442-53 (2007). IHC-P ; Mouse . PubMed: 17765889
