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    rb-phospho-s249-antibody-ab4788.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Rb
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Anti-Rb (phospho S249) 抗体 (ab4788)

  • Datasheet
Reviews (1)Q&A (1)References (2)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Rb (phospho S249) antibody (ab4788)

    Key features and details

    • Rabbit polyclonal to Rb (phospho S249)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    こちらの製品もご検討ください

    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    タンパク質
    Product image
    Recombinant Human Rb protein (ab83205)

    関連製品

    製品の概要

    • 製品名

      Anti-Rb (phospho S249) antibody
      Rb 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Rb (phospho S249)
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Chicken
    • 免疫原

      Synthetic peptide (Human) derived from a region of human Rb that contains serine 249 and threonine 252 (based on Swiss Protein database, accession number P06400). The sequence is conserved in human, mouse and rat.

    • ポジティブ・コントロール

      • Jurkat cells in high growth phase.
    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • バッファー

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. The final product is generated by affinity chromatography using a Rb-derived peptide that is phosphorylated at serine 249 and threonine 252.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • Rb
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Rb family

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant Human Rb protein (ab83205)

    アプリケーション

    Our Abpromise guarantee covers the use of ab4788 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB
  • 追加情報
    WB: 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 106 kDa).
    Can be blocked with Rb peptide - phospho S249 + T252 (phospho and non-phospho pair).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能

      Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
    • 組織特異性

      Expressed in the retina.
    • 関連疾患

      Childhood cancer retinoblastoma
      Bladder cancer
      Osteogenic sarcoma
    • 配列類似性

      Belongs to the retinoblastoma protein (RB) family.
    • ドメイン

      The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
    • 翻訳後修飾

      Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
      N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
      Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
    • 細胞内局在

      Nucleus.
    • Target information above from: UniProt accession P06400 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 386582 Chicken
      • Entrez Gene: 5925 Human
      • Entrez Gene: 19645 Mouse
      • Entrez Gene: 24708 Rat
      • Omim: 614041 Human
      • SwissProt: Q90600 Chicken
      • SwissProt: P06400 Human
      • SwissProt: P13405 Mouse
      • SwissProt: P33568 Rat
      • Unigene: 408528 Human
      • Unigene: 273862 Mouse
      • Unigene: 55115 Rat
      see all
    • 別名

      • Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
      • GOS563 exon 17 substitution mutation causes premature stop antibody
      • OSRC antibody
      • Osteosarcoma antibody
      • p105-Rb antibody
      • P105RB antibody
      • PP105 antibody
      • pp110 antibody
      • PPP1R130 antibody
      • pRb antibody
      • Prepro retinoblastoma associated protein antibody
      • Protein phosphatase 1 regulatory subunit 130 antibody
      • Rb antibody
      • RB transcriptional corepressor 1 antibody
      • RB_HUMAN antibody
      • RB1 antibody
      • RB1 gene antibody
      • Retinoblastoma 1 antibody
      • Retinoblastoma suspectibility protein antibody
      • Retinoblastoma-associated protein antibody
      see all

    画像

    • Western blot - Anti-Rb (phospho S249) antibody (ab4788)
      Western blot - Anti-Rb (phospho S249) antibody (ab4788)
      Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were incubated with 0.50 µg/mL ab4788, following prior incubation in the absence of the phosphopeptide immunogen (1), a generic phosphoserine containing peptide (2), a generic phosphothreonine containing peptide (3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (4), or the presence of the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of the ab4788 antibody for this epitope. Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 1

    プロトコール

    • Peptide Competition Experiment
    • Western blotting protocol

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (2)

    ab4788 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4788 は 2 報の論文で使用されています。

    • Pérez-Morales J  et al. Hyper-phosphorylation of Rb S249 together with CDK5R2/p39 overexpression are associated with impaired cell adhesion and epithelial-to-mesenchymal transition: Implications as a potential lung cancer grading and staging biomarker. PLoS One 13:e0207483 (2018). PubMed: 30452490
    • Lee YC  et al. Macrophage inflammatory protein-3alpha influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells. Toxicology 249:116-22 (2008). WB ; Human . PubMed: 18538911

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rb (phospho S249) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Jul 03 2018

    Question

    I have some problem with ab4788 (anti retinoblastoma) you send me two weeks ago. First of all it was supposed to be 50ul but it was only 20ul. Second, it doesn't detect any band even in the positive control (20ug of jurkat lysate, ab7899). I tried two times with two different incubations (2 hours at room temperature as you suggest in your protocol) and overnight at 4 degrees with no results. I hope you could resolve these problems.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 03 2005

    Answer

    Thank you for your email and I'm sorry to hear about the difficulty you are experiencing with ab4788. I apologize for the shortage that you received and can certainly send you a replacement vial free of charge. Can you please tell me the batch number that you received (it is located on the vial)? Also, what was the Abcam order number or purchase number that was used? Thank you, and I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    Answered on Feb 04 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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