RAW 264.7 whole cell lysate (ab7187)

Submit a review Q&A (1)References (5)

製品の概要

  • 製品名

    RAW 264.7 whole cell lysate

  • 特記事項

    Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor).
    Growth media: DMEM and 10% fetal bovine serum.

     

    Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.

  • アプリケーション

    適用あり: WBmore details

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Constituent: 100% SDS Sample Buffer
  • Concentration information loading...

  • ライゼート 備考

    Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 研究分野

  • 背景

    Established from the ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells have receptors for immunoglobulin and produce lysozyme. They are used in metabolic, inflammation and apoptosis studies.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7187の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent dilution. Ready to load.
特記事項
WB
Use at an assay dependent dilution. Ready to load.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (5)

ab7187 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7187 は 5 報の論文で使用されています。

  • Magalhaes RS  et al. A tissue-engineered uterus supports live births in rabbits. Nat Biotechnol 38:1280-1287 (2020). PubMed: 32601434
  • d'Uscio LV & Katusic ZS Vascular phenotype of amyloid precursor protein-deficient mice. Am J Physiol Heart Circ Physiol 316:H1297-H1308 (2019). PubMed: 30901278
  • Yao M  et al. CCR2 Chemokine Receptors Enhance Growth and Cell-Cycle Progression of Breast Cancer Cells through SRC and PKC Activation. Mol Cancer Res 17:604-617 (2019). PubMed: 30446625
  • Zaman RT  et al. Imaging cellular pharmacokinetics of 18F-FDG and 6-NBDG uptake by inflammatory and stem cells. PLoS One 13:e0192662 (2018). PubMed: 29462173
  • Madrigal-Matute J  et al. TWEAK/Fn14 interaction promotes oxidative stress through NADPH oxidase activation in macrophages. Cardiovasc Res 108:139-47 (2015). PubMed: 26224570

レビューと Q&A

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レビューを送る 質問を送る

Question

Dear Sir/Madam, We would like to know if this Anti-CPI17 alpha (phospho T38) antibody has been detected in C2C12 cellular extracts. If so, could you please send me some information regarding the detecting with this antibody. Looking forward to hearing from you.

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Answer

Thank you for contacting us.

We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells.

https://www.abcam.com/CPI-17-phospho-T38-antibody-ab52174.html&tab=abreviews&intabreviewid=13947

In his treatment, this customer used oxytocin, which we carry as ab120186 and phorbol 12-myristate 13-acetate (PMA), which we carry as ab120297.

https://www.abcam.com/Oxytocin-ab120186.html

https://www.abcam.com/Phorbol-12-myristate-13-acetate-PMA-ab120297.html

I do not know enough about the protein to tell you definitively whether or not this protein is endogenously phosphorylated in C2C12 cells. I do have an image of ab52174 used in WB with untreated RAW264.7 cells though, which we sell as ab7187. I'll be sure to get that WB image added to the datasheet online, but it will take a few days to appear.

https://www.abcam.com/RAW-264-7-Mouse-leukaemic-monocyte-macrophage-cell-line-Whole-Cell-Lysate-ab7187.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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