製品の概要

  • 製品名

    RAW 264.7 whole cell lysate
  • 特記事項

    Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor).
    Growth media: DMEM and 10% fetal bovine serum.


    Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション

    適用あり: WBmore details

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 精製度

    Whole Cell Lysate
  • ライゼート 備考

    Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究分野

  • 背景

    Established from the ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells have receptors for immunoglobulin and produce lysozyme. They are used in metabolic, inflammation and apoptosis studies.

アプリケーション

Our Abpromise guarantee covers the use of ab7187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Ready to load.

参考文献

This product has been referenced in:

  • d'Uscio LV & Katusic ZS Vascular phenotype of amyloid precursor protein-deficient mice. Am J Physiol Heart Circ Physiol 316:H1297-H1308 (2019). Read more (PubMed: 30901278) »
  • Zaman RT  et al. Imaging cellular pharmacokinetics of 18F-FDG and 6-NBDG uptake by inflammatory and stem cells. PLoS One 13:e0192662 (2018). Read more (PubMed: 29462173) »
See all 3 Publications for this product

レビューと Q&A

Answer

Thank you for contacting us.

We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells.

https://www.abcam.com/index.html?datasheet=52174&tab=abreviews&intabreviewid=13947

In his treatment, this customer used oxytocin, which we carry as ab120186 and phorbol 12-myristate 13-acetate (PMA), which we carry as ab120297.

https://www.abcam.com/Oxytocin-ab120186.html

https://www.abcam.com/Phorbol-12-myristate-13-acetate-PMA-ab120297.html

I do not know enough about the protein to tell you definitively whether or not this protein is endogenously phosphorylated in C2C12 cells. I do have an image of ab52174 used in WB with untreated RAW264.7 cells though, which we sell as ab7187. I'll be sure to get that WB image added to the datasheet online, but it will take a few days to appear.

https://www.abcam.com/RAW-264-7-Mouse-leukaemic-monocyte-macrophage-cell-line-Whole-Cell-Lysate-ab7187.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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