Rat uPA ELISA kit (total uPA antigen) (ab272781)

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Example data

    Key features and details

    • Range: 0.01 ng/ml - 10 ng/ml
    • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
    • Detection method: Colorimetric
    • Assay type: Quantitative
    • Reacts with: Rat

    製品の概要

    • 製品名

      Rat uPA ELISA kit (total uPA antigen)
      uPA キット 製品一覧

    • 検出方法

      Colorimetric

    • サンプルの種類

      Urine, Serum, Tissue Extracts, Cell culture media, EDTA Plasma, Cit plasma

    • アッセイタイプ

      Quantitative

    • 検出範囲

      0.01 ng/ml - 10 ng/ml

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Rat

    • 製品の概要

      Rat uPA ELISA kit (total uPA antigen) (ab272781) is intended for the quantitative determination of total urokinase plasminogen activator in rat plasma, serum, urine, cell culture media, or tissue extracts.


      Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.

    • アプリケーション

      適用あり: ELISAmore details

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at +4°C. Please refer to protocols.
    • 内容 1 x 96 tests
      10X Wash Buffer 1 x 50ml
      Anti-Rabbit Streptavidin-HRP Secondary Reagent 1 vial
      Anti-Rat uPA Primary Antibody Lyophilized Vial 1 vial
      Rat uPA ELISA Plate 1 unit
      Rat uPA Standard Lyophilized Vail 1 vial
      TMB Substrate 1 x 10ml

    • 研究分野

    • 関連性

      Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR).

    • 別名

      • ATF
      • BDPLT5
      • EC 3.4.21.73
      • PLAU
      • QPD
      • u PA
      • U plasminogen activator
      • u-PA
      • UPA
      • UPAM
      • Urinary plasminogen activator
      • URK
      • Urokinase plasminogen activator
      • Urokinase type plasminogen activator
      see all

    • 参照データベース

      関連製品

        アプリケーション

        The Abpromise guarantee

        Our Abpromise guarantee covers the use of ab272781 in the following tested applications.

        The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

        アプリケーション Abreviews 特記事項
        ELISA
        Use at an assay dependent concentration.
        特記事項
        ELISA
        Use at an assay dependent concentration.

        画像

        • Example data
          Example data

          A typical standard curve. Example only.

        参考文献 (0)

        ab272781 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

        ab272781 は論文での使用が確認できていません。

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