製品の概要

  • 製品名

    Rat Tissue Plasminogen Activator ELISA Kit (TPA)
    PAI1 + tPA キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Known Conc 20 0.8ng/ml 0.034 4.28%
    Known Conc 20 4.25ng/ml 0.225 5.29%
    Known Conc 20 13.2ng/ml 0.553 4.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Known Conc 10 1.37ng/ml 0.06 4.71%
    Known Conc 10 4.8ng/ml 0.34 7.13%
    Known Conc 10 13.3ng/ml 0.75 5.65%
  • サンプルの種類

    Plasma, Other biological fluids
  • アッセイタイプ

    Quantitative
  • 検出感度

    0.043 ng/ml
  • 検出範囲

    0.05 ng/ml - 50 ng/ml
  • 添加回収試験

    99 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Spike 99 93% - 107%

  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Rat
    非交差種: Sheep, Rabbit, Horse, Dog, Human
  • 製品の概要

    Abcam’s Tissue Plasminogen Activator (TPA) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of active tissue Plasminogen Activator (TPA) in rat plasma and other biological fluids.

     

    Functionally active rat TPA will form a covalent complex with the biotinylated Human PAI-1, which is bound to the avidin on the plate. Only free active TPA will react with the PAI-1 bound to the plate. After appropriate washing steps, polyclonal anti-TPA primary antibody binds to the captured TPA. Excess antibody is washed away and bound primary antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active TPA in the sample.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab198510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Full range standard curve using ab198510 Tissue Plasminogen Activator (TPA) Rat ELISA Kit.

  • Linear range standard curve using ab198510 Tissue Plasminogen Activator (TPA) Rat ELISA Kit.

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参考文献

This product has been referenced in:

  • Ito Y  et al. Generation and characterization of tissue-type plasminogen activator transgenic rats. J Thromb Thrombolysis 45:77-87 (2018). Read more (PubMed: 29168147) »
See 1 Publication for this product

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