Rat TIMP1 ELISA Kit (ab100782)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat TIMP1 ELISA Kit
TIMP1 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 4 pg/ml -
検出範囲
4.1 pg/ml - 3000 pg/ml -
添加回収試験
93 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 93.26 86% - 104% Serum 92.79 85% - 104% Plasma 94.65 84% - 103% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s TIMP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat TIMP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat TIMP- 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-rat TIMP1 2 vials Recombinant rat TIMP1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TIMP1 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻訳後修飾
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all -
参照データベース
- Entrez Gene: 116510 Rat
- SwissProt: P30120 Rat
- Unigene: 25754 Rat
画像
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Typical data B
Representative standard curve using ab100782
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Typical data ARepresentative standard curve using ab100782
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab100782 は論文での使用が確認できていません。