製品の概要

  • 製品名

    Rat TGF beta 1 ELISA Kit
    TGF beta 1 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Serum 4 6.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Plasma 4 6.8%
  • サンプルの種類

    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    8 pg/ml
  • 検出範囲

    31.3 pg/ml - 2000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 105 95% - 118%
    Serum 100 95% - 106%

  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Rat
  • 製品の概要

    Abcam’s TGF beta 1 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Rat TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA and heparin).

    Rat TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Rat TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rat TGF beta 1 captured on the plate.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Assay Buffer Concentrate 2 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Biotin-Conjugate anti-rat TGF beta 1 monoclonal antibody 1 x 120µl
    Microplate coated with monoclonal antibody to rat TGF beta 1 (12 x 8 wells) 1 unit
    Plate seal 6 units
    Rat TGF beta 1 Standard Lyophilized 2 vials
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究分野

  • 機能

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 組織特異性

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 関連疾患

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 配列類似性

    Belongs to the TGF-beta family.
  • 翻訳後修飾

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名

    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1
    • TGF beta 1 protein
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • Transforming growth factor beta 1
    • transforming growth factor beta-1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Activated TGF-beta 1 measured in tissue lysates, homogenised in RIPA buffer, activated (20 uL of HCl  per 20 uL of samples with 180 uL of buffer) and tested between 25-250 ug protein per mL. Data expressed as TGF beta per mg of extracted protein (duplicates +/- SD).

  • Activated TGF-beta 1 measured in rat biological fluids (dilution range 1/50-1/200; duplicates +/- SD). Human and mouse samples, diluted 1:12, gave results below the level of detection.

  • Sandwich ELISA on rat blood serum using Abcam's TGF beta 1 Rat ELISA Kit (ab119558).

  • Representative Standard Curve using ab119558.

プロトコール

参考文献

This product has been referenced in:

  • Wang QW  et al. Ginsenoside Re Improves Isoproterenol-Induced Myocardial Fibrosis and Heart Failure in Rats. Evid Based Complement Alternat Med 2019:3714508 (2019). Read more (PubMed: 30713572) »
  • Elimelech R  et al. Use of transforming growth factor-ß loaded onto ß-tricalcium phosphate scaffold in a bone regeneration rat calvaria model. Clin Implant Dent Relat Res N/A:N/A (2019). Read more (PubMed: 31025823) »
See all 11 Publications for this product

レビューと Q&A

1-8 of 8 Abreviews or Q&A

Answer

Thank you for contacting us.

Here is the correct protocol section:

Prepare your serum and plasma samples before starting the test
procedure. Dilute serum and plasma samples with Assay Buffer (1x)
according to the following scheme:
- 20 μl sample + 920 μl Assay Buffer (1x)
- Add 30 μl 1N HCl to 940 μl prediluted sample, mix and incubate for
1 hour at room temperature.
- Neutralize by addition of 30 μl 1N NaOH.
Prepare your cell culture supernatant samples before starting the test
procedure. Dilute cell culture supernatant samples with Assay Buffer
(1x) according to the following scheme:
- 20 μl sample + 180 μl Assay Buffer (1x)
- Add 20 μl 1N HCl to 200 μl prediluted sample, mix and incubate for
1 hour at room temperature.
- Neutralize by addition of 20 μl 1N NaOH.

The table is correct.

I am very sorry for this mistake and the confusion caused.
Please let me know how the results turned out.

If the data are incorrect or do not make any sense, please let me know your order or PO number as we made a mistake on our end and would like to remedy that by replacing the kit free of charge.
I look forward to hear back from you.

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Answer

We have not tested bronchoalveolar lavage fluid with ab119558, so we do not have a recommended dilution for this sample type. If you perform a literatire search to determine the expected levels of TGF beta 1 in this fluid compared to serum, plasma, or cell culture supernatant, then you can use the expected level as a guide to what dilution to use.

We always recommend testing multiple different dilutions of your sample to ensure that the readings fall within the range of the standard curve.

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Abreviews
TGF-beta ELISA assay kit in rat blood serum is very very excellent and easy step.
But, color development is very quickley disappears after stop solution.

Abcam user community

Verified customer

投稿 Mar 04 2014

Answer

Thank you for contacting us.


Our TGF beta 1 Rat ELISA Kit will detect the inactive ( which get activated during the sample preparation (HCL and NaOH)) and active form.

It will not detect the LAP complex.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting us.
I can confirm that the volume to be added is 400 ul.
The "2 x" is an oversight on our end due to the fact that 2 vials are being sent.
I will look into having this corrected.
Thank you for pointing this out to us.
Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you again for your patience.

I've just heard back from the lab, andstandard should be reconstituted in 400 ulto a concentration of400 pg/ml.

Please let me know if you have further questions or if there is anything else we can do. Have a great day.

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Question
Answer

Thank you for your call yesterday, and I apologize again for the missing protocol information.

I just wanted to touch base and let you know that I'm still waiting for this information from the lab. I will forward it on to you as soon as possible! Please let me know if there is anything else that we can do for you in the meantime, and we appreciate your patience.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. The estimated delivery date is xxx. I apologize about this delay.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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