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Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (Alkaline Phosphatase) preadsorbed (ab99602)

Submit a review Q&A (3)References (1)

Key features and details

  • Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (Alkaline Phosphatase) preadsorbed
  • Conjugation: Alkaline Phosphatase
  • Host species: Rat
  • Isotype: IgG2b
  • Suitable for: ELISA, Flow Cyt

Conjugates logo Related conjugates and formulations

Biotin FITC HRP PE Unconjugated

製品の概要

  • 製品名

    Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (Alkaline Phosphatase) preadsorbed
    IgG1 二次抗体 製品一覧

  • 由来種

    Rat

  • ターゲット生物種

    Mouse

  • アプリケーション

    適用あり: ELISA, Flow Cytmore details

  • 吸着処理血清


    Goat, Human, Rabbit more details

  • 標識

    Alkaline Phosphatase

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.0095% Magnesium chloride, 0.605% Tris, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    IgG fraction

  • ポリ/モノ

    モノクローナル

  • クローン名

    SB77e

  • アイソタイプ

    IgG2b

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab99602の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/500 - 1/1000.
Flow Cyt
Use at an assay dependent dilution.
特記事項
ELISA
1/500 - 1/1000.
Flow Cyt
Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab99602 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab99602 は 1 報の論文で使用されています。

  • Sircar G  et al. Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant: A CANDIDATE MOLECULE FOR FUNGAL ALLERGY VACCINES. J Biol Chem 291:18016-29 (2016). PubMed: 27358405

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

Inquiry: Hi, I'm wondering what the nucleotide sequence is of the peroxidase is conjugated to this product ab127427

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Answer

The calf intestinal alkaline phosphatase conjugated to ab127427 is purified from healthy bovine material. It is not a recombinant protein.

I hope this is helpful. Let us know if you have any questions or if we may assist you further.

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Question

Sorry I made a mistake, I meant ab99602. A rat monoclonal secondary to mouse IgG1 conjugated to Alkaline Phosphatase.

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Answer

Thanks for your reply, although I'm afraid I still don't understand what information you are looking for. Are you looking for the sequence of the gene that expresses the alkaline phosphatase that is conjugated to ab99602?

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Question

We have recently purchased several antibodies from abcam. I'm the end user and was wondering if you possibly could supply a little more information about above products.

I have set up an assay to detect biotinylated DNA using your anti-biotin Ms antibody: ab79111. I detect a splendid shift on acrylamide gel when working with an oligo duplex but when working with larger DNA on agarose gel I don't see a nice band-to-band, instead it does look like a smear. Is this expected or should I assume the antibody is slowly coming off the biotinylated DNA during electrophoresis?
Do you perhaps know what is the binding constant between biotin and this antibody? How does it compare to biotin-streptavidin, I'm sure it's weaker but not sure how much. Do you know what is the overall charge of this protein and how many lysines does it contain?

My second question deals with your product: ab99602. Is concentration value available? I also wanted to check what is the molecule charge. What kind of binding should I expect between ab 79111 and ab99602, even a ballpark would be useful? Mostly do you expect it to be stronger than between epitope and primary antibody or weaker?

I'm nor very experienced working with antibodies and would greatly appreciate your feedback.

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Answer

Thank you for contacting Abcam.

I am not familiar with the assay that you are using and so I am not sure if I can answer your first question, but I will speak to some of my colleagues and see if there is anything they can suggest. I will let you know if I find out anything.

As for the second question, we do not have the concentration for this product available and I would expect that the binding between the antibodies would be as strong as that between the epitope and primary antibody.

Sorry I could not provide more information, but I will ask my colleagues and get back to you if I find anything else out.

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