Rat IL-2 ELISA Kit (ab221834)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.9 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture media, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IL-2 ELISA Kit
IL-2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 3 4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 5 4.2% -
サンプルの種類
Serum, Cell culture media, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
4.9 pg/ml -
検出範囲
31.25 pg/ml - 2000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 93 89% - 100% Tissue Extracts 45 43% - 48% Cell culture media 108 99% - 113% Cit plasma 86 84% - 88% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat -
製品の概要
Rat IL-2 ELISA Kit (ab221834) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-2 protein in serum, cell culture media, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-2 with 4.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical monomeric polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, natural killer cell (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, thereby regulating cytokine cascades that correlate with each of the Th differentiation states. Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases.
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試験プラットフォーム
Microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Rat IL-2 Capture Antibody 1 x 600µl 10X Rat IL-2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Rat IL-2 Lyophilized Recombinant Protein 2 vials Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
関連疾患
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
配列類似性
Belongs to the IL-2 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all -
参照データベース
- Entrez Gene: 116562 Rat
- SwissProt: P17108 Rat
- Unigene: 9871 Rat
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat IL-2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat IL-2 standard curve in Sample Diluent 60BS. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen (2 days) supernatant samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 6401 pg/mL in Concanavalin A treated rat spleen supernatant.
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Interpolated concentrations of spiked IL-2 in rat serum and plasma samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Comparison of IL-2 in unstimulated and Concanavalin A stimulated rat spleen supernatants. Rat spleen was cultured for 2 days in the presence or absence of 5 µg/mL Concanavalin A. The concentrations of IL-2 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-2 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-2 concentration was determined to be 6042 pg/mL in Concanavalin A stimulated neat rat spleen supernatant and undetectable in unstimulated rat spleen supernatant and RPMI media (not shown).
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab221834 は 3 報の論文で使用されています。
- Alkhedaide AQ Anti-inflammatory Effect of Juniperus Procera Extract in Rats Exposed to Streptozotocin Toxicity. Antiinflamm Antiallergy Agents Med Chem 18:71-79 (2019). PubMed: 30474537
- Alam MF et al. Thymoquinone Ameliorates Doxorubicin-Induced Cardiotoxicity in Swiss Albino Mice by Modulating Oxidative Damage and Cellular Inflammation. Cardiol Res Pract 2018:1483041 (2018). PubMed: 29805796
- Sun Y et al. Reduction of Uterine Perfusion Pressure Induced Redistribution of Endothelin Receptor Type-B Between the Intima and Media Contributes to the Pathogenesis of Pregnancy-Induced Hypertension. Cell Physiol Biochem 44:1715-1725 (2017). PubMed: 29216636