製品の概要

  • 製品名

    Rat IL-17F ELISA Kit
    IL-17F キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample 1 16 275pg/ml 12.93 4.7%
    Sample 2 16 524pg/ml 32.5 6.2%
    Sample 3 16 928pg/ml 52 5.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample 1 24 352pg/ml 17.6 5%
    Sample 2 24 723pg/ml 52.78 7.3%
    Sample 3 24 1230pg/ml 83.64 6.8%
  • サンプルの種類

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 10 pg/ml
  • 検出範囲

    31.2 pg/ml - 2000 pg/ml
  • 全工程の試験時間

    3h 30m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Rat
  • 製品の概要

    The Rat IL-17F Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213913) is designed for the quantitative measurement of Rat     IL-17F in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17F has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for IL-17F is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-17F amount of sample captured in plate.

  • 特記事項

    Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM-CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF. 

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-rat IL-17F coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- rat IL-17F antibody 1 x 130µl
    Lyophilized recombinant rat IL-17F standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • 研究分野

  • 機能

    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
  • 組織特異性

    Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
  • 関連疾患

    Candidiasis, familial, 6
  • 配列類似性

    Belongs to the IL-17 family.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • CANDF6
    • Cytokine ML 1
    • Cytokine ML-1
    • IL 17F
    • IL 24
    • IL-17F
    • IL-24
    • Il17f
    • IL17F_HUMAN
    • Interleukin 17F
    • Interleukin 24
    • Interleukin-17F
    • Interleukin-24
    • ML 1
    • ML1
    • Mutant IL 17F
    • OTTHUMP00000016602
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab213913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Rat IL-17F ELISA Kit (ab213913) Standard Curve

プロトコール

参考文献

ab213913 has not yet been referenced specifically in any publications.

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