製品の概要

  • 製品名

    Rat IL-17A ELISA Kit
    IL-17A キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3 3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 5 7%
  • サンプルの種類

    Cell culture supernatant, Serum, Citrate Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    1.1 pg/ml
  • 検出範囲

    6.25 pg/ml - 400 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 113 105% - 117%
    Cell culture media 101 96% - 110%
    Citrate Plasma 110 108% - 112%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human
  • 製品の概要

    IL-17A in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A protein in rat serum, plasma, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS: 1.1 pg/mL
    Samples in Sample Diluent 75BS: 3.2 pg/mL

  • 特記事項

    IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Rat IL-17a Capture Antibody 1 x 600µl
    10X Rat IL-17a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BR 1 x 6ml
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Rat IL-17a Lyophilized Recombinant Protein 2 vials
    Sample Diluent 75BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野

  • 機能

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • 組織特異性

    Restricted to activated memory T-cells.
  • 配列類似性

    Belongs to the IL-17 family.
  • 翻訳後修飾

    Found both in glycosylated and nonglycosylated forms.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • interleukin17
    • Interleukin17A
    • OTTHUMP00000016597
    • OTTMUSP00000046003
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab214028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Rat spleen cells were cultured in the absence or presence of 5 µg/mL Concanavalin A for 48 hours. The concentrations of Il-17a were measured in neat supernatant samples in duplicates and interpolated from the IL-17a standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean IL‑17a concentration was determined to be 33.2 pg/mL in unstimulated, 187 pg/mL in Concanavalin A stimulated supernatants and undetectable in media (not shown).

  • The concentrations of IL-17a were measured in duplicates, interpolated from the IL-17a standard curves and corrected for sample dilution. Undiluted samples are as follows: rat spleen cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17a concentration was determined to be 190 pg/mL in Concanavalin A treated rat spleen cell culture supernatant.

  • The concentrations of IL-17a were measured in duplicates, interpolated from the IL-17a standard curves and corrected for sample dilution. Undiluted samples are as follows: rat serum and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Background-subtracted data values (n = 1) are graphed. Note that this kit is specific for IL-17a homodimer, it does not recognize IL-17f homodimer or IL-17a/f heterodimer.

  • Two fold serial dilutions of rat, mouse and human recombinant IL-17a proteins were measured with this kit. Background-subtracted data values (mean +/- SD, n = 2) are graphed. O.D. values for human IL-17a protein were below background values. Note this kit reacts with mouse recombinant IL-17a protein. Note this kit does not react with human recombinant IL-17a protein.

  • Background-subtracted data values (mean +/- SD) are graphed

プロトコール

参考文献

This product has been referenced in:

  • Hu X  et al. RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll-like receptor signaling pathway. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 31012109) »
  • Feng F  et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). ELISA ; Rat . Read more (PubMed: 30546393) »
See all 2 Publications for this product

レビューと Q&A

simplestep

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit is very easy to use, the whole process takes less than 2 hours to produce results, and the operation is very simple, much easier than I have used a conventional ELISA kit. This kit is highly recommended.

Abcam user community

Verified customer

投稿 Aug 09 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録