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Rat IL-13 ELISA Kit (ab269547)

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SimpleStep ELISA Protocol Diagram
  • Example of rat IL-13 standard curve in Sample Diluent 50BP.
  • Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.
  • Sandwich ELISA - Rat IL-13 ELISA Kit (ab269547)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 20.03 pg/ml
  • Range: 78.13 pg/ml - 5000 pg/ml
  • Sample type: Cell culture media, EDTA Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

製品の概要

  • 製品名

    Rat IL-13 ELISA Kit
    IL-13 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Serum 8 5.9%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Serum 3 2%

  • サンプルの種類

    Urine, Serum, Cell culture media, EDTA Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    20.03 pg/ml

  • 検出範囲

    78.13 pg/ml - 5000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Urine 83 80% - 86%
    Serum 87 81% - 92%
    Cell culture media 90 84% - 98%
    EDTA Plasma 97 84% - 117%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Rat

  • 製品の概要

    Rat IL-13 ELISA Kit (ab269547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-13 protein in edta plasma, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-13 with 20.03 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL-2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure. The antibodies for this kit were raised against a recombinant peptide fragment of the mature rat protein.

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

画像

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of rat IL-13 standard curve in Sample Diluent 50BP.
    Example of rat IL-13 standard curve in Sample Diluent 50BP.

    The IL-13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.
    Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.

    The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: rat serum 50%, rat plasma (EDTA) 50%, rat urine 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Rat IL-13 ELISA Kit (ab269547)
    Sandwich ELISA - Rat IL-13 ELISA Kit (ab269547)
    To learn more about the advantages of recombinant antibodies see here.

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