Rat IL-10 ELISA Kit (ab214566)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.89 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IL-10 ELISA Kit
IL-10 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 8 5.6% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 3.4% -
サンプルの種類
Cell culture supernatant, Serum, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
4.89 pg/ml -
検出範囲
31.3 pg/ml - 2000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 93 84% - 109% Serum 94 93% - 94% Cit plasma 86 85% - 89% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat
非交差種: Cow -
製品の概要
Rat IL-10 ELISA Kit (ab214566) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-10 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-10 with 4.89 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Interleukin-10 is an anti-inflammatory cytokine involved in inhibition of a variety of other cytokines, including IFN-gamma, TNF, IL-2 and GM-CSF.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 10X Rat IL-10 Capture Antibody 1 x 600µl 10X Rat IL-10 Detector Antibody 1 x 600µl Rat IL-10 Lyophilized Recombinant Protein 2 vials Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
参照データベース
- Entrez Gene: 25325 Rat
- SwissProt: P29456 Rat
- Unigene: 9868 Rat
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows: rat PBMC stimulated with PHA (10 µg/mL, 48 hours) supernatant 50%, unstimulated PBMC supernatant 100%, and spleen stimulated with concanavalin A (5 µg/mL, 48 hours) supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 1,174 pg/mL in PBMC stimulated with PHA supernatant, 230 pg/mL in unstimulated PBMC supernatant and 789 pg/mL in spleen stimulated with concanavalin A supernatant.
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Standard curve comparison between rat IL-10 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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Conditioned media was harvested after 48 hours. IL-10 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-10 were measured in duplicate, interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 1,068 pg/mL in PHA stimulated PBMC supernatant and 227 pg/mL in unstimulated PBMC supernatant.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab214566 は 21 報の論文で使用されています。
- Huang X et al. Histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit exacerbates inflammation in depression rats by modulating microglia polarization. Bioengineered 13:5509-5524 (2022). PubMed: 35172677
- Mohamed ME et al. The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NF?B Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326185
- Park YJ et al. Effects of Glucocorticoid Therapy on Sepsis Depend Both on the Dose of Steroids and on the Severity and Phase of the Animal Sepsis Model. Life (Basel) 12:N/A (2022). PubMed: 35330172
- Zhang K et al. Sevoflurane attenuates hepatic ischemia reperfusion injury by the miR-122/Nrf2 pathway. Ann Transl Med 10:350 (2022). PubMed: 35433991
- Chen B et al. Hirudin Regulates Vascular Function in Chronic Renal Failure through Modulating Macrophage Polarization. Biomed Res Int 2022:6043698 (2022). PubMed: 35496058