Rat IL-10 ELISA Kit (ab100765)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IL-10 ELISA Kit
IL-10 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall < 10% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall < 12% -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
8.23 pg/ml - 6000 pg/ml -
添加回収試験
140 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture extracts 142.1 133% - 149% Tissue Extracts 138.7 127% - 146% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s IL-10 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-10 in cell lysates and tissue lysates.
This assay employs an antibody specific for rat IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 140X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Rat IL-10 2 vials IL-10 Microplate (12 strips x 8 wells) 1 unit Recombinant rat IL-10 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
参照データベース
- Entrez Gene: 25325 Rat
- SwissProt: P29456 Rat
- Unigene: 9868 Rat
画像
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Typical Standard Curve
Representative standard curve using ab100765.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab100765 は 18 報の論文で使用されています。
- Li Y et al. Protective effect of CTRP6 on cerebral ischemia/reperfusion injury by attenuating inflammation, oxidative stress and apoptosis in PC12 cells. Mol Med Rep 22:344-352 (2020). PubMed: 32377750
- Yang W et al. Sulforaphene Ameliorates Neuroinflammation and Hyperphosphorylated Tau Protein via Regulating the PI3K/Akt/GSK-3ß Pathway in Experimental Models of Alzheimer's Disease. Oxid Med Cell Longev 2020:4754195 (2020). PubMed: 32963694
- Tu C et al. Ginsenoside Rg3 alleviates inflammation in a rat model of myocardial infarction via the SIRT1/NF-?B pathway. Exp Ther Med 20:238 (2020). PubMed: 33193843
- Lu HK et al. Pinellia ternata attenuates carotid artery intimal hyperplasia and increases endothelial progenitor cell activity via the PI3K/Akt signalling pathway in wire-injured rats. Pharm Biol 58:1184-1191 (2020). PubMed: 33253601
- Hellenbrand DJ et al. Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury. J Neuroinflammation 16:93 (2019). PubMed: 31039819