We tested IL-1β levels in homogenized rat spinal cord. The results had to high of background and the CV values were extremely high. The tech help from ABCAM was very helpful. We tried several of their recommendations including using a blocker, different dilutions, centrifuging again before adding samples, and triplicate wells, but nothing helped. It’s possible this will work for other sample types, but I would not recommend it for spinal cord. Note the high CV values in the image.
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投稿 Mar 15 2020