Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)

Q&A (5)References (6)

製品の概要

  • 製品名

    Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control
    Rat アイソタイプ・コントロール 製品一覧

  • 特記事項（標識）

    Please note that this antibody is unconjugated.

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Frmore details

  • 免疫原

    Trinitrophenol + KLH

  • 特記事項

    ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS

    0.2 µm filter sterilized
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    The immunoglobulin was purified by affinity chromatography.

  • アイソタイプ・コントロール 備考

    ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • ポリ/モノ

    モノクローナル

  • クローン名

    RTK2758

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Rat Isotype Control

アプリケーション

Our Abpromise guarantee covers the use of ab18450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells.

ab18450 is a negative isotype control.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

画像

  • Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
    Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)

    Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

参考文献 (6)

ab18450 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18450 は 6 報の論文で使用されています。

  • Shi Q  et al. The identification of the ATR inhibitor VE-822 as a therapeutic strategy for enhancing cisplatin chemosensitivity in esophageal squamous cell carcinoma. Cancer Lett 432:56-68 (2018). PubMed: 29890208
  • Chang TZ  et al. Effects of ovalbumin protein nanoparticle vaccine size and coating on dendritic cell processing. Biomater Sci 5:223-233 (2017). PubMed: 27918020
  • Daschkey S  et al. MicroRNAs distinguish cytogenetic subgroups in pediatric AML and contribute to complex regulatory networks in AML-relevant pathways. PLoS One 8:e56334 (2013). IP . PubMed: 23418555
  • Doi T  et al. IgA plasma cells express the negative regulatory co-stimulatory molecule programmed cell death 1 ligand and have a potential tolerogenic role in the intestine. Biochem Biophys Res Commun 425:918-23 (2012). Blocking . PubMed: 22906740
  • Hausmann M  et al. BCL-2 Modifying Factor (BMF) Is a Central Regulator of Anoikis in Human Intestinal Epithelial Cells. J Biol Chem 286:26533-40 (2011). PubMed: 21673109
  • Coughlan L  et al. In vivo retargeting of adenovirus type 5 to alphavbeta6 integrin results in reduced hepatotoxicity and improved tumor uptake following systemic delivery. J Virol 83:6416-28 (2009). Flow Cyt . PubMed: 19369326

レビューと Q&A

Q&A
質問を送る

1-5 of 5 Q&A

Question

Provide the discount code for ab18407 and ab18541

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Answer

Expiration date: 25-04-2012
I am very pleased to hear you would like to use ab18407 and ab18541 in ICC/IF and send us an image of your results.
This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview with the image for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.
If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

I might be interested in purchasing the anti-IL-17RB monoclonal
(ab86488).
The extracellular domain of the IL17Rb receptor is said to have been
used for generating the antibody, however only western blot is listed
for its application.
I was wondering whether it has been tested for flow cytometry and it
doesn't work so it is not listed or whether it hasn't been
tested for this application yet?

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Answer

Thank you for your patience.

The subisotype ofab86488 is IgG2a. Thus, the recommended isotype control to be used in flow cytometry would be ab18450.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I might be interested in purchasing the anti-IL-17RB monoclonal
(ab86488).
The extracellular domain of the IL17Rb receptor is said to have been
used for generating the antibody, however only western blot is listed
for its application.
I was wondering whether it has been tested for flow cytometry and it
doesn't work so it is not listed or whether it hasn't been
tested for this application yet?

Read More
Answer

Thank you for contacting us.

Flow cytometry has not yet been tested with this antibody. Therefore, we do not have data to show if and how well it would work in this application. But for UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


As isotype control, I would suggest either

ab18450 (rat IgG2a) or ab18541 (rat IgG2b)
https://www.abcam.com/Rat-IgG2a-kappa-monoclonal-aRTK2758-ab18450.html
https://www.abcam.com/Rat-IgG2b-kappa-monoclonal-RTK4530-Isotype-control-ab18541.html

I will check with the lab if they have more information about the specific subisotype of this antibody (2a or 2b).



Please let me know if I shall issue a testing discount code for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Purchased wrong antibody ab18703 and ab18450. How could we return the antibodies? We are interested in getting ab18446 and rat monoclonal anti IgG2c antibody which we could not find in catalogue.

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Answer

Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today. - Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet). Please note that we will be unable to issue the credit note if the product is dispatched without the cooler box. However we are not able to accept products returned on dry ice. - Please mark on the documents the reason of return and Ref: - Please return using an over night courier. - You will need to provide the courier with an AirWay Bill – which you can get from the courier, and fill out with our details. - Our address is: Abcam plc, 330 Cambridge Science Park, Milton Road, Cambridge, CB4 0FL When we have received the goods, we will process the return. We will then raise a credit note for the invoice less 20% restocking fee. If you have already paid this invoice, we will reallocate this money to a new invoice for a future order and if there’s any difference in price, we will refund or re-invoice accordingly. For the correct item, please place a new order in the normal methods. Please confirm if you are planning to return the product to us. Important: if the product is not returned to Abcam within next 7 days we will cancel the credit note. PS: We have anti IgG2a and anti IgG2c available in catalogue that are currently in stock. These 2 antibodies exactly match your request. The catalogue numbers are: ab18446 and ab114134

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Question

I wanted to know if this antibody has been used in-vivo before in mice.

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Answer

Thank you for your enquiry. To our knowledge this antibody has yet to be tested in-vivo. If you decide to purchase this antibody for testing in applications for which we don't have any information, please let us know how you get on. In return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

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