アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Isotype/Loading Controls Isotype Controls Rat
Share by email

Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)

  • Datasheet
  • SDS
Q&A (5)References (12)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)

    Conjugates logo Related conjugates and formulations

    Biotin FITC

    こちらの製品もご検討ください

    アイソタイプ
    Product image
    Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    アイソタイプ
    Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
    一次抗体
    Biotin Anti-V5 tag antibody [SV5-Pk1] (ab27672)

    関連製品

    製品の概要

    • 製品名

      Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control
      Rat アイソタイプ・コントロール 製品一覧
    • 特記事項(標識)

      Please note that this antibody is unconjugated.
    • アプリケーション

      適用あり: ICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Frmore details
    • 免疫原

      Trinitrophenol + KLH

    • 特記事項

      ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • バッファー

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: PBS

      0.2 µm filter sterilized
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      The immunoglobulin was purified by affinity chromatography.
    • アイソタイプ・コントロール 備考

      ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
    • ポリ/モノ

      モノクローナル
    • クローン名

      RTK2758
    • アイソタイプ

      IgG2a
    • 軽鎖の種類

      kappa
    • 研究分野

      • Isotype/Loading Controls
      • Isotype Controls
      • Rat
    • 別名

      • Rat Isotype Control

    関連製品

    • Alternative Versions

      • Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18445)
      • FITC Rat IgG2a, kappa monoclonal [RTK2758] - Isotype control (ab18446)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab18450の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt
    Use 2µg for 106 cells.

    ab18450 is a negative isotype control.

    IP
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    特記事項
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt
    Use 2µg for 106 cells.

    ab18450 is a negative isotype control.

    IP
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.

    画像

    • Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
      Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)

      Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    プロトコール

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (12)

    ab18450 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab18450 は 12 報の論文で使用されています。

    • Santini MP  et al. Tissue-Resident PDGFRa+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner. Cell Rep 30:555-570.e7 (2020). PubMed: 31940496
    • Saraswati S  et al. Small proline-rich repeat 3 is a novel coordinator of PDGFRß and integrin ß1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts. FASEB J 34:7885-7904 (2020). PubMed: 32297675
    • Chang HR  et al. Role of LpL (Lipoprotein Lipase) in Macrophage Polarization In Vitro and In Vivo. Arterioscler Thromb Vasc Biol 39:1967-1985 (2019). PubMed: 31434492
    • Hordeaux J  et al. The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain Barrier. Mol Ther 27:912-921 (2019). PubMed: 30819613
    • Mantani PT  et al. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol 20:47 (2019). PubMed: 31823769
    View all Publications for this product

    レビューと Q&A

    Q&A
    質問を送る

    1-5 of 5 Q&A

    Question

    Provide the discount code for ab18407 and ab18541

    Read More

    Abcam community

    Verified customer

    Asked on Apr 25 2012

    Answer

    Expiration date: 25-04-2012
    I am very pleased to hear you would like to use ab18407 and ab18541 in ICC/IF and send us an image of your results.
    This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview with the image for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.
    If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Apr 25 2012

    Question

    I might be interested in purchasing the anti-IL-17RB monoclonal
    (ab86488).
    The extracellular domain of the IL17Rb receptor is said to have been
    used for generating the antibody, however only western blot is listed
    for its application.
    I was wondering whether it has been tested for flow cytometry and it
    doesn't work so it is not listed or whether it hasn't been
    tested for this application yet?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 02 2012

    Answer

    Thank you for your patience.

    The subisotype ofab86488 is IgG2a. Thus, the recommended isotype control to be used in flow cytometry would be ab18450.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 02 2012

    Question

    I might be interested in purchasing the anti-IL-17RB monoclonal
    (ab86488).
    The extracellular domain of the IL17Rb receptor is said to have been
    used for generating the antibody, however only western blot is listed
    for its application.
    I was wondering whether it has been tested for flow cytometry and it
    doesn't work so it is not listed or whether it hasn't been
    tested for this application yet?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 02 2012

    Answer

    Thank you for contacting us.

    Flow cytometry has not yet been tested with this antibody. Therefore, we do not have data to show if and how well it would work in this application. But for UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


    As isotype control, I would suggest either

    ab18450 (rat IgG2a) or ab18541 (rat IgG2b)
    https://www.abcam.com/Rat-IgG2a-kappa-monoclonal-aRTK2758-ab18450.html
    https://www.abcam.com/Rat-IgG2b-kappa-monoclonal-RTK4530-Isotype-control-ab18541.html

    I will check with the lab if they have more information about the specific subisotype of this antibody (2a or 2b).



    Please let me know if I shall issue a testing discount code for you.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 02 2012

    Question

    Purchased wrong antibody ab18703 and ab18450. How could we return the antibodies? We are interested in getting ab18446 and rat monoclonal anti IgG2c antibody which we could not find in catalogue.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 24 2011

    Answer

    Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today. - Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet). Please note that we will be unable to issue the credit note if the product is dispatched without the cooler box. However we are not able to accept products returned on dry ice. - Please mark on the documents the reason of return and Ref: - Please return using an over night courier. - You will need to provide the courier with an AirWay Bill – which you can get from the courier, and fill out with our details. - Our address is: Abcam plc, 330 Cambridge Science Park, Milton Road, Cambridge, CB4 0FL When we have received the goods, we will process the return. We will then raise a credit note for the invoice less 20% restocking fee. If you have already paid this invoice, we will reallocate this money to a new invoice for a future order and if there’s any difference in price, we will refund or re-invoice accordingly. For the correct item, please place a new order in the normal methods. Please confirm if you are planning to return the product to us. Important: if the product is not returned to Abcam within next 7 days we will cancel the credit note. PS: We have anti IgG2a and anti IgG2c available in catalogue that are currently in stock. These 2 antibodies exactly match your request. The catalogue numbers are: ab18446 and ab114134

    Read More

    Abcam Scientific Support

    Answered on Aug 24 2011

    Question

    I wanted to know if this antibody has been used in-vivo before in mice.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 31 2005

    Answer

    Thank you for your enquiry. To our knowledge this antibody has yet to be tested in-vivo. If you decide to purchase this antibody for testing in applications for which we don't have any information, please let us know how you get on. In return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

    Read More

    Abcam Scientific Support

    Answered on Mar 31 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.