Rat IFN gamma ELISA Kit (ab46107)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 31.25 pg/ml - 1000 pg/ml
    • Sample type: Cell culture supernatant, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

    製品の概要

    • 製品名

      Rat IFN gamma ELISA Kit
      Interferon gamma キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      A 19 365.1pg/ml 12.7 2.9%
      B 20 554.6pg/ml 16.4 3.4%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      A 6 148.2pg/ml 6.6 4.5%
      B 6 560.7pg/ml 29.5 5.2%

    • サンプルの種類

      Cell culture supernatant, Serum

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      < 10 pg/ml

    • 検出範囲

      31.25 pg/ml - 1000 pg/ml

    • 全工程の試験時間

      3h 45m

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Rat

    • 製品の概要

      Abcam’s IFN gamma Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in cell culture supernatants, buffered solutions and plasma.

      A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

      This kit will recognize both endogenous and recombinant rat IFN gamma

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at +4°C. Please refer to protocols.
    • 内容 ラベル 1 x 96 tests 2 x 96 tests
      10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
      200X Wash Buffer White 1 x 10ml 2 x 10ml
      Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
      Biotinylated anti-IFN gamma Red 1 x 400µl 1 x 400µl
      Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
      HRP Diluent Red 1 x 23ml 1 x 23ml
      IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units
      IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials
      Stop Reagent Black 1 x 11ml 2 x 11ml
      Streptavidin-HRP 2 x 5µl 4 x 5µl

    • 研究分野

    • 機能

      Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

    • 組織特異性

      Released primarily from activated T lymphocytes.

    • 関連疾患

      In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

    • 配列類似性

      Belongs to the type II (or gamma) interferon family.

    • 翻訳後修飾

      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 別名

      • IF 1
      • IFG
      • IFI
      • IFN gamma
      • IFN immune
      • IFN, immune
      • IFN-gamma
      • IFNG
      • IFNG_HUMAN
      • Immune interferon
      • Interferon gamma
      • Type II Interferon
      see all

    • 参照データベース

      関連製品

        画像

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab46107

        参考文献 (8)

        ab46107 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

        ab46107 は 8 報の論文で使用されています。

        • Sun J  et al. Cytoprotective effects of galacto-oligosaccharides on colon epithelial cells via up-regulating miR-19b. Life Sci 231:116589 (2019). PubMed: 31226416
        • Chen YX  et al. Artesunate exerts protective effects against ulcerative colitis via suppressing Toll-like receptor 4 and its downstream nuclear factor-?B signaling pathways. Mol Med Rep 20:1321-1332 (2019). PubMed: 31173225
        • Eftekhar N  et al. Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma. BMC Complement Altern Med 19:349 (2019). PubMed: 31801507
        • Saad DY  et al. Protective effects of sea cucumber (Holothuria atra) extract on testicular dysfunction induced by immune suppressant drugs in Wistar rats. Andrologia N/A:e13017 (2018). PubMed: 29687464
        • Feng F  et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). PubMed: 30546393
        • Yang Y  et al. The Anti-Inflammatory Effect of Guchangzhixie-Pill by Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in an Ulcerative Colitis Rat Model. Evid Based Complement Alternat Med 2017:8547257 (2017). PubMed: 28845184
        • Dolatabadi HRD  et al. The Effects of Pentoxifylline on Serum Levels of Interleukin 10 and Interferon Gamma and Memory Function in Lipopolysaccharide-induced Inflammation in Rats. Adv Biomed Res 6:110 (2017). PubMed: 28904938
        • Payghani C  et al. The Effect of Levothyroxine on Serum Levels of Interleukin 10 and Interferon-gamma in Rat Model of Multiple Sclerosis. Adv Biomed Res 6:118 (2017). PubMed: 28989911

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