製品の概要

  • 製品名

  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 19 365.1pg/ml 12.7 2.9%
    B 20 554.6pg/ml 16.4 3.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 6 148.2pg/ml 6.6 4.5%
    B 6 560.7pg/ml 29.5 5.2%
  • サンプルの種類

    Cell culture supernatant, Serum
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 10 pg/ml
  • 検出範囲

    31.25 pg/ml - 1000 pg/ml
  • 全工程の試験時間

    3h 45m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Rat
  • 製品の概要

    Abcam’s IFN gamma Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in cell culture supernatants, buffered solutions and plasma.

    A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

    This kit will recognize both endogenous and recombinant rat IFN gamma

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IFN gamma Red 1 x 400µl 1 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units
    IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野

  • 機能

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 組織特異性

    Released primarily from activated T lymphocytes.
  • 関連疾患

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • 配列類似性

    Belongs to the type II (or gamma) interferon family.
  • 翻訳後修飾

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • IF 1
    • IFG
    • IFI
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46107

プロトコール

参考文献

This product has been referenced in:

  • Saad DY  et al. Protective effects of sea cucumber (Holothuria atra) extract on testicular dysfunction induced by immune suppressant drugs in Wistar rats. Andrologia N/A:e13017 (2018). Read more (PubMed: 29687464) »
  • Feng F  et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). Read more (PubMed: 30546393) »
See all 5 Publications for this product

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